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Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.
Open accessAbstract
We describe an efficient method for generating female germline mosaics by inducing site-specific homologous mitotic recombination with a yeast recombinase (FLP) which is driven by a heat shock promoter. These germline mosaics are produced in flies heterozygous for the agametic, germline-dependent, dominant female sterile (DFS) mutation ovoD1, where only flies possessing germline clones are able to lay eggs. This method, the "FLP-DFS" technique, is very efficient because more than 90% of females with germline clones can be recovered. We show that this heat-inducible, site-specific mitotic recombination system does not affect viability and that the germline clones recovered are physiologically the same as those created by X-ray induced mitotic recombination. We describe the parameters of FLP-recombinase induced germline mitotic recombination and the use of the "FLP-DFS" technique to analyze the maternal effect of X-linked zygotic lethal mutations.
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Citations
Cited by
Axelrod, J. D., Miller, J. R., Shulman, J. M., Moon, R. T., & Perrimon, N. (1998) · Genes & Development · doi:10.1101/gad.12.16.2610 · Cited by 659
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Struhl, G., Fitzgerald, K., & Greenwald, I. (1993) · Cell · doi:10.1016/0092-8674(93)90424-o · Cited by 462
Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts
Wodarz, A., Ramrath, A., Kuchinke, U., & Knust, E. (1999) · Nature · doi:10.1038/990128 · Cited by 451
Peifer, M., Sweeton, D., Casey, M., & Wieschaus, E. (1994) · Development · doi:10.1242/dev.120.2.369 · Cited by 426
folded gastrulation , cell shape change and the control of myosin localization
Dawes-Hoang, R. E., Parmar, K. M., Christiansen, A. E., Phelps, C. B., Brand, A. H., & Wieschaus, E. F. (2005) · Development · doi:10.1242/dev.01938 · Cited by 414
Metadata
- Authors
- Chou, T. B., & Perrimon, N.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1992-07-01
- Volume
- 131
- Issue
- 3
- Pages
- 643-653
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2155615473
- Cited by
- 483
- References
- 0
- FWCI
- 8.44
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Chou, T. B., & Perrimon, N. (1992). Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila. Genetics. https://doi.org/10.1093/genetics/131.3.643
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