The DOI matches its Crossref record; 89 of the 89 references it cites were found in a registry or an index.
Essential Functional Interactions of SAGA, a Saccharomyces cerevisiae Complex of Spt, Ada, and Gcn5 Proteins, With the Snf/Swi and Srb/Mediator Complexes
Abstract
The Saccharomyces cerevisiae transcription factor Spt20/Ada5 was originally identified by mutations that suppress Ty insertion alleles and by mutations that suppress the toxicity caused by Gal4-VP16 overexpression. Here we present evidence for physical associations between Spt20/Ada5 and three other Spt proteins, suggesting that they exist in a complex. A related study demonstrates that this complex also contains the histone acetyltransferase, Gcn5, and Ada2. This complex has been named SAGA (Spt/Ada/Gcn5 acetyltransferase). To identify functions that genetically interact with SAGA, we have screened for mutations that cause lethality in an spt20Δ/ada5Δ mutant. Our screen identified mutations in SNF2, SIN4, and GAL11. These mutations affect two known transcription complexes: Snf/Swi, which functions in nucleosome remodeling, and Srb/mediator, which is required for regulated transcription by RNA polymerase II. Systematic analysis has demonstrated that spt20Δ/ada5Δ and spt7Δ mutations cause lethality with every snf/swi and srb/mediator mutation tested. Furthermore, a gcn5Δ mutation causes severe sickness with snf/swi mutations, but not with srb/mediator mutations. These findings suggest that SAGA has multiple activities and plays critical roles in transcription by RNA polymerase II.
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Metadata
- Authors
- Roberts, S. M., & Winston, F.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-10-01
- Volume
- 147
- Issue
- 2
- Pages
- 451-465
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1954246782
- Cited by
- 278
- References
- 89
- FWCI
- 11.92
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Essential Functional Interactions of SAGA, a Saccharomyces cerevisiae Complex of Spt, Ada, and Gcn5 Proteins, With the Snf/Swi and Srb/Mediator Complexes. https://app.cochat.ai/papers/10.1093/genetics/147.2.451
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- Roberts, S. M., & Winston, F. (1997). Essential Functional Interactions of SAGA, a Saccharomyces cerevisiae Complex of Spt, Ada, and Gcn5 Proteins, With the Snf/Swi and Srb/Mediator Complexes. Genetics. https://doi.org/10.1093/genetics/147.2.451
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