The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Optimal and consistent protein glycosylation in mammalian cell culture
Abstract
In the biopharmaceutical industry, mammalian cell culture systems, especially Chinese hamster ovary (CHO) cells, are predominantly used for the production of therapeutic glycoproteins. Glycosylation is a critical protein quality attribute that can modulate the efficacy of a commercial therapeutic glycoprotein. Obtaining a consistent glycoform profile in production is desired due to regulatory concerns because a molecule can be defined by its carbohydrate structures. An optimal profile may involve a spectrum of product glycans that confers a desired therapeutic efficacy, or a homogeneous glycoform profile that can be systemically screened for. Studies have shown some degree of protein glycosylation control in mammalian cell culture, through cellular, media, and process effects. Studies upon our own bioprocesses to produce fusion proteins and monoclonal antibodies have shown an intricate relationship between these variables and the resulting protein quality. Glycosylation optimization will improve therapeutic efficacy and is an ongoing goal for researchers in academia and industry alike. This review will focus on the advancements made in glycosylation control in a manufacturing process, as well as the next steps in understanding and controlling protein glycosylation.
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Cites
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Cited by
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
Xu, X., Nagarajan, H., Lewis, N. E., Pan, S., Cai, Z., Liu, X., Chen, W., Xie, M., Wang, W., Hammond, S., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1932 · Cited by 785
Recent advances in large-scale production of monoclonal antibodies and related proteins
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Expression systems for therapeutic glycoprotein production
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Trends in Upstream and Downstream Process Development for Antibody Manufacturing
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Therapeutic glycoprotein production in mammalian cells
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Metadata
- Authors
- Hossler, P., Khattak, S. F., & Li, Z. J.
- Venue
- Glycobiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-06-03
- Volume
- 19
- Issue
- 9
- Pages
- 936-949
- ISSN
- 0959-6658 (ISSN-L)
- 1460-2423
- OpenAlex
- W2146981871
- Cited by
- 669
- References
- 10
- FWCI
- 12.72
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hossler, P., Khattak, S. F., & Li, Z. J. (2009). Optimal and consistent protein glycosylation in mammalian cell culture. Glycobiology. https://doi.org/10.1093/glycob/cwp079
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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