Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions
Abstract
Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structural studies of the galactomannan of Aspergillus fumigatus, some inconsistencies are present in these results. In this study, to clarify the relationship between the growth conditions and structure of the galactomannans, we cultured A. fumigatus using two distinct yeast/fungal cultivation media, i.e. the yeast extract-peptone-dextrose (YPD) medium and yeast nitrogen base (YNB) medium. Galactomannans prepared from the resulting culture supernatants were structurally characterized by (1)H and (13)C nuclear magnetic resonance, methylation analysis, acetolysis and α-mannosidase degradation. These assays revealed that the galactomannan from the YPD cultivation had short β-1,5-linked galactofuranose (Galf) oligosaccharide chains in both the O- and N-linked carbohydrate moieties, while the galactomannan from the YNB cultivation incorporated long Galf oligosaccharide chains. The galactomannans derived from the two culture conditions significantly differed in reactivity based on the EIA diagnostic system. We also demonstrated the presence of a novel Galf-containing branched oligosaccharide in the O-linked moiety.
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Expanding the catalogue of epitopes recognized can explain the occurrence of some false-positive tests obtained with enzyme immunoassays using EB-A2 MAb.
“Expanding the catalogue of epitopes recognized can explain the occurrence of some false-positive tests obtained with enzyme immunoassays (EIAs) using EB-A2 MAb (121, 122).”
From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [121]
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Aspergillus fumigatus and Aspergillosis in 2019
Latgé, J.-P., & Chamilos, G. (2019) · Clinical Microbiology Reviews · doi:10.1128/cmr.00140-18 · Cited by 1,400 · see citing works
Krylov, V. B., Argunov, D. A., Solovev, A. S., Petruk, M. I., Gerbst, A. G., Dmitrenok, A. S., Shashkov, A. S., Latgé, J.-P., & Nifantiev, N. E. (2018) · Organic & Biomolecular Chemistry · doi:10.1039/c7ob02734f · Cited by 51 · see citing works
- Authors
- Kudoh, A., Okawa, Y., & Shibata, N.
- Affiliations
- Sendai City Medical Center (JP)
- Tohoku Medical and Pharmaceutical University (JP)
- Corresponding author
- Shibata, Nobuyuki
- Venue
- Glycobiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-09-03
- Volume
- 25
- Issue
- 1
- Pages
- 74-87
- ISSN
- 0959-6658 (ISSN-L)
- 1460-2423
- OpenAlex
- W2137553641
- Cited by
- 64
- References
- 45
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 5 · 2023: 6 · 2022: 2 · 2021: 6 · 2020: 9 · 2019: 8 · 2018: 7 · 2017: 4 · 2016: 5 · 2015: 1
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Glycobiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Aspergillus fumigatus, galactomannan, invasive aspergillosis, N-linked carbohydrates, culture medium effects, YPD medium, enzyme immunoassay, antigenic polysaccharide, branched oligosaccharides
- MeSH terms
- Antigens, Fungal, Aspergillus fumigatus, Carbohydrate Conformation, Carbohydrate Sequence, Culture Media, Galactose, Hydrolysis, Immunoenzyme Techniques, Mannans, Methylation, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, alpha-Mannosidase, Fungal Polysaccharides
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