Cited by 64Glycosylation and Glycoproteins ResearchAntifungal resistance and susceptibilityFungal and yeast genetics research

Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions

Kudoh, Atsushi, Okawa, Yoshio, Shibata, Nobuyuki·2015·Glycobiology

Abstract

Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structural studies of the galactomannan of Aspergillus fumigatus, some inconsistencies are present in these results. In this study, to clarify the relationship between the growth conditions and structure of the galactomannans, we cultured A. fumigatus using two distinct yeast/fungal cultivation media, i.e. the yeast extract-peptone-dextrose (YPD) medium and yeast nitrogen base (YNB) medium. Galactomannans prepared from the resulting culture supernatants were structurally characterized by (1)H and (13)C nuclear magnetic resonance, methylation analysis, acetolysis and α-mannosidase degradation. These assays revealed that the galactomannan from the YPD cultivation had short β-1,5-linked galactofuranose (Galf) oligosaccharide chains in both the O- and N-linked carbohydrate moieties, while the galactomannan from the YNB cultivation incorporated long Galf oligosaccharide chains. The galactomannans derived from the two culture conditions significantly differed in reactivity based on the EIA diagnostic system. We also demonstrated the presence of a novel Galf-containing branched oligosaccharide in the O-linked moiety.

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  • Expanding the catalogue of epitopes recognized can explain the occurrence of some false-positive tests obtained with enzyme immunoassays using EB-A2 MAb.

    “Expanding the catalogue of epitopes recognized can explain the occurrence of some false-positive tests obtained with enzyme immunoassays (EIAs) using EB-A2 MAb (121, 122).”

    From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [121]

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Authors
Kudoh, A., Okawa, Y., & Shibata, N.
Affiliations
  • Sendai City Medical Center (JP)
  • Tohoku Medical and Pharmaceutical University (JP)
Corresponding author
Shibata, Nobuyuki
Venue
Glycobiology
Published
2014-09-03
Volume
25
Issue
1
Pages
74-87
ISSN
  • 0959-6658 (ISSN-L)
  • 1460-2423
OpenAlex
W2137553641
Cited by
64
References
45
Citations by year
2026: 6 · 2025: 5 · 2024: 5 · 2023: 6 · 2022: 2 · 2021: 6 · 2020: 9 · 2019: 8 · 2018: 7 · 2017: 4 · 2016: 5 · 2015: 1
FWCI
1.37
Type
journal article
Language
en
Open access
closed
Locations
  • Glycobiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
Aspergillus fumigatus, galactomannan, invasive aspergillosis, N-linked carbohydrates, culture medium effects, YPD medium, enzyme immunoassay, antigenic polysaccharide, branched oligosaccharides
MeSH terms
Antigens, Fungal, Aspergillus fumigatus, Carbohydrate Conformation, Carbohydrate Sequence, Culture Media, Galactose, Hydrolysis, Immunoenzyme Techniques, Mannans, Methylation, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, alpha-Mannosidase, Fungal Polysaccharides

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