A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis
Open accessAbstract
A double-direct sandwich enzyme-linked immunosorbent assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoassay, which detects less than 1 ng of galactomannan per ml, was assessed in a retrospective study with samples from patients with invasive aspergillosis. Serum is more appropriate than urine for use in the search for circulating galactomannan. Antigenemia does not have a transient character. Galactomannan can be detected at least 39 days before the death of the patients.
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- Authors
- Stynen, D., Goris, A., Sarfati, J., & Latgé, J. P.
- Affiliations
- Sanofi (Belgium) (BE)
- ORCID
- Latgé, J P
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1995-02
- Volume
- 33
- Issue
- 2
- Pages
- 497-500
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC227977
- OpenAlex
- W2123332089
- Cited by
- 330
- References
- 20
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 4 · 2023: 2 · 2022: 5 · 2021: 7 · 2020: 5 · 2019: 13 · 2018: 9 · 2017: 6 · 2016: 6 · 2015: 6 · 2014: 9 · 2013: 8 · 2012: 14
- FWCI
- 7.46
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- galactomannan, invasive aspergillosis, sandwich ELISA, serum biomarkers, antigenemia, Aspergillus, fungal infection diagnosis
- MeSH terms
- Adolescent, Adult, Antigens, Fungal, Aspergillosis, Aspergillus, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, False Positive Reactions, Female, Galactose, Humans, Male, Mannans, Middle Aged, Opportunistic Infections, Sensitivity and Specificity
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