Cited by 267Antifungal resistance and susceptibilityFungal and yeast genetics researchFungal Infections and Studies

Rat monoclonal antibodies against Aspergillus galactomannan

Open access
Stynen, D, Sarfati, J, Goris, A, Prévost, M C, Lesourd, M, Kamphuis, H, Darras, V, Latgé, J P·1992·Infection and Immunity

Abstract

Monoclonal antibodies (MAbs) against Aspergillus fumigatus galactomannan were produced in rats. Seven of them, EB-A1 through EB-A7, were characterized in more detail. They were all immunoglobulin M antibodies, reacting in an indirect enzyme-linked immunosorbent assay with purified A. fumigatus galactomannan, with avidity constants of between 2 x 10(9) and 5 x 10(9)/M. Enzyme-linked immunosorbent assay inhibition experiments with modified galactomannan and synthetic oligomers of beta (1----5)galactofuranose demonstrated that the MAbs bound to an epitope located on the beta(1----5)galactofuranose-containing side chains of the galactomannan molecule. An identical or similar epitope also seemed to be present in other fungi. Immunofluorescence and immunoelectron microscopy experiments with EB-A2 revealed the presence of the antigen in the fungal wall and inside the cell. Immunoblotting experiments demonstrated that the epitope recognized by the MAbs was a common oligosaccharide moiety of a wide range of intracellular and extracellular glycoproteins in A. fumigatus. The characteristics of the MAbs justify their use in the diagnosis of invasive aspergillosis by antigen detection.

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Registry

Crossref · 10.1128/iai.60.6.2237-2245.1992

Cites

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  • The method currently used for GM detection is a sandwich ELISA based on a very efficient EB-A2 rat monoclonal antibody able to detect 0.5 to 1 ng of the galactofuran epitope.

    “The method currently used is a sandwich ELISA based on a very efficient EB-A2 rat monoclonal antibody able to detect 0.5 to 1 ng of the galactofuran epitope (107, 108).”

    From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [107]

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Authors
Stynen, D., Sarfati, J., Goris, A., Prévost, M. C., Lesourd, M., Kamphuis, H., Darras, V, & Latgé, J. P.
Affiliations
Sanofi (Belgium) (BE)
Venue
Infection and Immunity
Published
1992-06
Volume
60
Issue
6
Pages
2237-2245
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
OpenAlex
W1915036617
Cited by
267
References
47
Citations by year
2026: 3 · 2025: 7 · 2024: 3 · 2023: 4 · 2022: 7 · 2021: 7 · 2020: 5 · 2019: 19 · 2018: 8 · 2017: 8 · 2016: 8 · 2015: 5 · 2014: 7 · 2013: 9 · 2012: 11
FWCI
10.53
Type
journal article
Language
en
Open access
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • Lirias (submittedVersion)
  • Rivm Repository (Netherlands National Institute for Public Health and the Environment) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
Aspergillus fumigatus, galactomannan, monoclonal antibodies, fungal cell wall, antigen detection, fungal glycoproteins, epitope mapping, IgM
MeSH terms
Animals, Antibodies, Monoclonal, Epitopes, Antigens, Fungal, Aspergillus, Cross Reactions, Fluorescent Antibody Technique, Galactose, Mannans, Immunoblotting, Rats

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