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Cytoskeleton proteins are modulators of mutant tau-induced neurodegeneration in Drosophila
Abstract
Tauopathies, including Alzheimer's disease and fronto-temporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), are a group of neurodegenerative disorders characterized by the presence of intraneuronal filamentous inclusions of aberrantly phosphorylated-tau. Tau is a neuronal microtubule-associated protein involved in microtubule assembly and stabilization. Currently, the molecular mechanisms underlying tau-mediated cellular toxicity remain elusive. To address the determinants of tau neurotoxicity, we first characterized the cellular alterations resulting from the over-expression of a mutant form of human tau associated with FTDP-17 (tau V337M) in Drosophila. We found that the over-expression of tau V337M, in Drosophila larval motor neurons, induced disruption of the microtubular network at presynaptic nerve terminals and changes in neuromuscular junctions morphological features. Secondly, we performed a misexpression screen to identify genetic modifiers of the tau V337M-mediated rough eye phenotype. The screening of 1250 mutant Drosophila lines allowed us to identify several components of the cytoskeleton, and particularly from the actin network, as specific modifiers of tau V337M-induced neurodegeneration. Furthermore, we found that numerous tau modulators identified in our screen were involved in the maintenance of synaptic function. Taken together, these findings suggest that disruption of the microtubule network in presynaptic nerve terminals could constitute early events in the pathological process leading to synaptic dysfunction in tau V337M pathology.
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“many investigators have shown that either inhibition or genetic reduction of GSK-3β ameliorates tau-related deficits (94–99).”
From Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation (2011) · cites this paper as [98]
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“Here we report novel modifiers distinct from those identified in two recent reports that also screened for genetic modifiers of tau-induced neurodegeneration in Drosophila (52,98).”
From Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation (2011) · cites this paper as [98]
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Yang, P., Liu, H., Li, Y., Gao, Q., Chen, X., Chang, J., Li, Y., Chen, S., Dong, R., Wu, H., et al. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.959832 · Cited by 8
Codon-optimized TDP-43 mediates neurodegeneration in a Drosophila model of ALS/FTLD
Yusuff, T., Chatterjee, S., Chang, Y., Sang, T.-K., & Jackson, G. R. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.881638 · Cited by 6
Mouchel‐Vielh, E., Bloyer, S., Salvaing, J., Randsholt, N. B., & Peronnet, F. (2008) · Genes to Cells · doi:10.1111/j.1365-2443.2008.01231.x · Cited by 7
Identification of Tau Toxicity Modifiers in the Drosophila Eye
Dourlen, P. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-6598-4_26 · Cited by 8
The Biology and Pathobiology of Tau Protein
Hall, G. F. (2012) · Humana Press eBooks · doi:10.1007/978-1-61779-788-0_15 · Cited by 6
Khani, M., Nafissi, S., Shamshiri, H., Moazzeni, H., Taheri, H., Sadeghi, M., Salehi, N., Chitsazian, F., & Elahi, E. (2022) · European Journal of Neurology · doi:10.1111/ene.15528 · Cited by 3
Drosophila Models of Tauopathy
Ali, Y., Ruan, K., & Zhai, R. G. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-405195-9.00054-8 · Cited by 2
Insights into amyloid disease from fly models
Chen, K., & Crowther, D. C. (2014) · Essays in Biochemistry · doi:10.1042/bse0560069 · Cited by 2
Codon-optimized TDP-43-mediated neurodegeneration in a Drosophila model for ALS/FTLDPreprint
Yusuff, T., Chatterjee, S., Chang, Y., Sang, T., & Jackson, G. R. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/696963 · Cited by 2
MeDUsA: A novel system for automated axon quantification to evaluate neuroaxonal degenerationPreprint
Nitta, Y., Kawai, H., Osaka, J., Hakeda‐Suzuki, S., Nagai, Y., Doubková, K., Suzuki, T., Tavosanis, G., & Sugie, A. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.10.25.465674 · Cited by 2
Prefoldin 5 is a microtubule-associated protein that suppresses Tau aggregation and neurotoxicity
Bisht, A., Pippadpally, S., Majumder, S., Gopi, A. T., Das, A., Sahi, C., Ramaswami, M., & Kumar, V. (2024) · eLife · doi:10.7554/elife.104691.3 · Cited by 4
Sergeant, N., & Buée, L. (2010) · Advances in neurobiology · doi:10.1007/978-1-4419-6787-9_4 · Cited by 1
Neurodegeneration models in Drosophila
Roy, B., & Jackson, G. R. (2014) · Cambridge University Press eBooks · doi:10.1017/cbo9780511920585.012 · Cited by 1
Experimental Models of Tau Aggregation
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Elliott, J. L., & Yin, J. C. P. (2007) · Future Neurology · doi:10.2217/14796708.2.4.389 · Cited by 1
Butzlaff, M. (2011) · eDiss (Georg-August-Universität Göttingen) · doi:10.53846/goediss-1007
Metadata
- Authors
- Blard, O., Feuillette, S., Bou, J., Chaumette, B., Frébourg, T., Campion, D., & Lecourtois, M.
- Affiliations
- Inserm (FR)
- Corresponding author
- Lecourtois, Magalie
- Venue
- Human Molecular Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-02-19
- Volume
- 16
- Issue
- 5
- Pages
- 555-566
- ISSN
- 0964-6906 (ISSN-L)
- 1460-2083
- OpenAlex
- W2112207786
- Cited by
- 110
- References
- 45
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 7 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 3 · 2016: 6 · 2015: 8 · 2014: 9 · 2013: 9 · 2012: 8
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Molecular Genetics (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Sanofi
- Région Normandie
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Clinical Neurology › Amyotrophic Lateral Sclerosis Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- tauopathy, FTDP-17, cytoskeleton, actin networks, presynaptic terminals, neuromuscular junction, synaptic dysfunction, genetic modifiers, rough eye phenotype, Alzheimer's disease
- MeSH terms
- Animals, Cytoskeletal Proteins, Cytoskeleton, Drosophila melanogaster, Eye, Female, Humans, Larva, Male, Methionine, Nerve Degeneration, Neuromuscular Junction, Peptides, Phenotype, Phosphorylation, Valine, tau Proteins, Molecular Chaperones, Mutant Proteins
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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