The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
DNA methylation and gene expression differences in children conceived in vitro or in vivo
Open accessAbstract
Epidemiological data indicate that children conceived in vitro have a greater relative risk of low birth-weight, major and minor birth defects, and rare disorders involving imprinted genes, suggesting that epigenetic changes may be associated with assisted reproduction. We examined DNA methylation at more than 700 genes (1536 CpG sites) in placenta and cord blood and measured gene expression levels of a subset of genes that differed in methylation levels between children conceived in vitro versus in vivo. Our results suggest that in vitro conception is associated with lower mean methylation at CpG sites in placenta and higher mean methylation at CpG sites in cord blood. We also find that in vitro conception-associated DNA methylation differences are associated with gene expression differences at both imprinted and non-imprinted genes. The range of inter-individual variation in gene expression of the in vitro and in vivo groups overlaps substantially but some individuals from the in vitro group differ from the in vivo group mean by more than two standard deviations. Several of the genes whose expression differs between the two groups have been implicated in chronic metabolic disorders, such as obesity and type II diabetes. These findings suggest that there may be epigenetic differences in the gametes or early embryos derived from couples undergoing treatment for infertility. Alternatively, assisted reproduction technology may have an effect on global patterns of DNA methylation and gene expression. In either case, these differences or changes may affect long-term patterns of gene expression.
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Cites
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Perinatal outcomes in singletons following in vitro fertilization: a meta–analysis
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The Risk of Major Birth Defects after Intracytoplasmic Sperm Injection and in Vitro Fertilization
Hansen, M., Kurinczuk, J. J., Bower, C., & Webb, S. (2002) · New England Journal of Medicine · doi:10.1056/nejmoa010035 · Cited by 1,083
Low and Very Low Birth Weight in Infants Conceived with Use of Assisted Reproductive Technology
Schieve, L. A., Meikle, S. F., Ferre, C., Peterson, H. B., Jeng, G., & Wilcox, L. S. (2002) · New England Journal of Medicine · doi:10.1056/nejmoa010806 · Cited by 1,017
Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture
Young, L. E., Fernandes, K., McEvoy, T. G., Butterwith, S. C., Gutierrez, C. G., Carolan, C., Broadbent, P. J., Robinson, J. J., Wilmut, I., & Sinclair, K. D. (2001) · Nature Genetics · doi:10.1038/84769 · Cited by 829
CpG methylation, chromatin structure and gene silencing---a three-way connection
Razin, A. (1998) · The EMBO Journal · doi:10.1093/emboj/17.17.4905 · Cited by 794
Differential Effects of Culture on Imprinted H19 Expression in the Preimplantation Mouse Embryo1
Doherty, A. S., Mann, M. R. W., Tremblay, K. D., Bartolomei, M. S., & Schultz, R. M. (2000) · Biology of Reproduction · doi:10.1095/biolreprod62.6.1526 · Cited by 722
Assisted reproductive technologies and the risk of birth defects—a systematic review
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High-throughput DNA methylation profiling using universal bead arrays
Bibikova, M., Lin, Z., Zhou, L., Chudin, E., Garcia, E. W., Wu, B., Doucet, D., Thomas, N. J., Wang, Y., Vollmer, E., et al. (2006) · Genome Research · doi:10.1101/gr.4410706 · Cited by 657
Self-citationWidespread Monoallelic Expression on Human Autosomes
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Cited by
Sandovici, I., Hoelle, K., Angiolini, E., & Constância, M. (2012) · Reproductive BioMedicine Online · doi:10.1016/j.rbmo.2012.03.017 · Cited by 224
DNA methylation in metabolic disorders
Barres, R., & Zierath, J. R. (2011) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.110.001933 · Cited by 174
Paternal Influences on Offspring Development: Behavioural and Epigenetic Pathways
Braun, K., & Champagne, F. A. (2014) · Journal of Neuroendocrinology · doi:10.1111/jne.12174 · Cited by 157
Imprinted genes and the epigenetic regulation of placental phenotype
Fowden, A. L., Coan, P. M., Angiolini, E., Burton, G. J., & Constancia, M. (2011) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2010.11.005 · Cited by 142
Argyraki, M., Damdimopoulou, P., Chatzimeletiou, K., Grimbizis, G. F., Tarlatzis, B. C., Syrrou, M., & Lambropoulos, A. (2019) · Human Reproduction Update · doi:10.1093/humupd/dmz025 · Cited by 86
Metadata
- Authors
- Katari, S., Turan, N., Bibikova, M., Erinle, O., Chalian, R., Foster, M., Gaughan, J. P., Coutifaris, C., & Sapienza, C.
- Venue
- Human Molecular Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-07-15
- Volume
- 18
- Issue
- 20
- Pages
- 3769-3778
- ISSN
- 0964-6906 (ISSN-L)
- 1460-2083
- OpenAlex
- W2170408710
- Cited by
- 470
- References
- 41
- FWCI
- 18.24
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Katari, S., Turan, N., Bibikova, M., Erinle, O., Chalian, R., Foster, M., Gaughan, J. P., Coutifaris, C., & Sapienza, C. (2009). DNA methylation and gene expression differences in children conceived in vitro or in vivo. Human Molecular Genetics. https://doi.org/10.1093/hmg/ddp319
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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