The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells
Abstract
BACKGROUND: Human embryonic stem (hES) cell lines were first cultured using fetal mouse fibroblasts as feeder cells. To avoid feeders and to reduce the amount of xeno-components, Matrigel- and laminin-coated dishes, and conditioned mouse feeder cell medium have been used, and hES cells have also been cultured on human fetal muscle and skin, and adult Fallopian tube epithelial cells. METHODS: We used post-natal, commercially available human foreskin fibroblasts as feeder cells. Inner cell masses (ICM) were isolated from five supernumerary blastocysts, obtained as donations from couples undergoing IVF treatment. RESULTS: Two ICM showed continuous growth. One line, HS181, has been in culture for 41 weeks with a doubling time of 24-36 h. It continues to express stem cell markers alkaline phosphatase, Oct-4, stage-specific embryonic antigen (SSEA)-4 and tumour-related antigen (TRA)-1-60. The karyotype is 46,XX. Pluripotency was demonstrated by teratoma formation in immunodeficient mice. In high-density cultures, spontaneous differentiation to beating cells and neuron-like cells was seen. The second line, HS207, was cultured for 9 weeks and cryopreserved, as were samples of line HS181. Both lines began to grow after thawing. CONCLUSIONS: We used successfully human foreskin fibroblasts as feeder cells for derivation and continued undifferentiated growth of hES cells. These feeder cells are convenient for IVF units, because no fetal human tissues or tissue from operations are needed.
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Cited by
Human embryonic stem cells express an immunogenic nonhuman sialic acid
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Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell
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Direct conversion of human fibroblasts to dopaminergic neurons
Pfisterer, U., Kirkeby, A., Torper, O., Wood, J., Nelander, J., Dufour, A., Björklund, A., Lindvall, O., Jakobsson, J., & Parmar, M. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1105135108 · Cited by 744
Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
Rodin, S., Domogatskaya, A., Ström, S., Hansson, E. M., Chien, K. R., Inzunza, J., Hovatta, O., & Tryggvason, K. (2010) · Nature Biotechnology · doi:10.1038/nbt.1620 · Cited by 553
Self-citationCharacterization and culture of human embryonic stem cells
Hoffman, L. M., & Carpenter, M. K. (2005) · Nature Biotechnology · doi:10.1038/nbt1102 · Cited by 465
Metadata
- Authors
- Hovatta, O.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-07-01
- Volume
- 18
- Issue
- 7
- Pages
- 1404-1409
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2154040966
- Cited by
- 494
- References
- 14
- FWCI
- 13.08
- Type
- journal article
- Language
- en
- Open access
- closed
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