Prolonged HCG action affects angiogenic substances and improves follicular maturation, oocyte quality and fertilization competence in patients with polycystic ovarian syndrome
Abstract
BACKGROUND: The aim of this study was to determine whether, in polycystic ovarian syndrome (PCOS) patients, HCG action prolonged for 4 h improves the action of angiogenic substances [ovarian renin angiotensin system and vascular endothelial growth factor (VEGF)], and consequently follicular maturation, oocyte quality and oocyte fertilization competence. METHODS: In this prospective study 20 patients with PCOS undergoing IVF were included. Oocyte retrieval was carried out either 34 or 38 h after HCG administration. Each follicle was analysed for prorenin, active renin, VEGF and estradiol. Oocytes were evaluated for quality (mature, immature, degenerated oocytes), as were the embryos (low or high). RESULTS: In the HCG +38 h group there were 245 follicles, and in the HCG +34 h group 240 follicles. In the HCG +38 h group, log active renin was lower (2.78 +/- 0.20 versus 2.91 +/- 0.25; P < 0.001) and VEGF higher (2276.0 +/- 790.1 versus 1946.6 +/- 954.5 pg/ml; P < 0.001). The odds ratio for obtaining oocytes from follicles was 1.6 [95% confidence interval (CI) 1.1-2.6; P = 0.02], and for developing high quality embryos 7.6 (95% CI 2.8-20.9; P < 0.001) in favour of the HCG +38 h group. CONCLUSIONS: Follicular maturation and oocyte quality are related to the intrafollicular influences of active renin and VEGF in a time-dependent manner after HCG administration, whereas fertilization competence is related to VEGF only.
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Renin levels in hFF differ in a variety of ovarian diseases or abnormalities.
“Renin levels in hFF also show differently in a variety of ovarian diseases or abnormalities according to a large number of proteomic analyses [36–38]”
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干润新, Huang, X., Zhao, J., Zhang, Q., Huang, C., & Li, Y. (2023) · Figshare · doi:10.6084/m9.figshare.23621418
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- Authors
- Bokal, E. V., Vrtovec, H. M., Klun, I. V., & Verdenik, I.
- Affiliations
- Ljubljana University Medical Centre (SI)
- Corresponding author
- Bokal, E. Vrtačnik
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-06-01
- Volume
- 20
- Issue
- 6
- Pages
- 1562-1568
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2119984741
- Cited by
- 69
- References
- 49
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 13 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 1
- FWCI
- 2.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
- Keywords
- polycystic ovary syndrome, human chorionic gonadotropin, hCG administration timing, follicle maturation, oocyte quality, fertilization competence, renin-angiotensin system, vascular endothelial growth factor, oocyte retrieval, embryo quality, in vitro fertilization
- MeSH terms
- Adult, Embryo, Mammalian, Estradiol, Female, Fertilization in Vitro, Chorionic Gonadotropin, Ovarian Follicle, Humans, Infertility, Female, Oocytes, Polycystic Ovary Syndrome, Pregnancy, Renin, Follicular Fluid, Sperm Injections, Intracytoplasmic, Vascular Endothelial Growth Factor A, Angiogenic Proteins
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