Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis
Abstract
BACKGROUND: Endometriosis occurs in 10% of women and is currently diagnosed by invasive laparoscopic testing. We tested the hypothesis that endometrial gene expression in late secretory phase endometrium differs between patients with and without endometriosis. METHODS: Ten patients with laparoscopically proven endometriosis (minimal/mild n = 5 and moderate/severe n = 5) and six controls, underwent endometrial biopsy in the late secretory phase (Day 23 onwards). Microarray interrogation of eutopic endometrial gene expression was performed. RESULTS: Microarray data were obtained for all control samples and eight samples from the endometriosis patients (n = 4 minimal/mild, n = 4 moderate/severe disease). Eight genes were identified as up-regulated and one gene was down-regulated in all endometriotic samples (more than 1.75-fold, P < 0.01). Real-time PCR analysis of protocadherin-17 (PCDH17), protein tyrosine phosphatase, receptor type, R (PTPRR) and interleukin-6 signal transducer (IL6ST) expression validated the microarray findings. CONCLUSIONS: Expression of very few transcripts differs, in late secretory eutopic endometrium, between controls and patients with endometriosis. The median fold changes of these genes are small. No transcripts were identified that could discriminate between minimal/mild and moderate/severe endometriosis. Therefore, interrogation of the late secretory endometrial transcriptome is not likely to form the basis of a minimally invasive diagnostic test for endometriosis.
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Significant differences in gene expression exist in eutopic endometrium from women with versus without endometriosis at the transcript level.
“At the transcript level, significant differences in gene expression exist in eutopic endometrium from women with versus without endometriosis [114–117].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [117]
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Self-citation
- Authors
- Sherwin, J. R. A., Sharkey, A. M., Mihalyi, A., Simsa, P., Catalano, R. D., & D'Hooghe, T. M.
- Affiliations
- Rosie Hospital (GB)
- University of Cambridge (GB)
- Universitair Ziekenhuis Leuven (BE)
- ORCID
- Sharkey, A.M.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-05-01
- Volume
- 23
- Issue
- 5
- Pages
- 1063-1068
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2091963543
- Cited by
- 66
- References
- 32
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 2 · 2020: 2 · 2019: 1 · 2018: 5 · 2017: 7 · 2016: 3 · 2015: 5 · 2014: 3 · 2013: 2 · 2012: 6
- FWCI
- 9.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Cambridge
- Biotechnology and Biological Sciences Research Council (8/EGH16 106)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Health Sciences › Medicine › Obstetrics and Gynecology › Gynecological conditions and treatments
- Keywords
- endometriosis, eutopic endometrium, endometrial gene expression, endometrial transcriptome, minimally invasive diagnosis, PTPRR, gp130, endometriosis severity
- MeSH terms
- Endometriosis, Endometrium, Female, Humans, Luteal Phase, Down-Regulation, Cadherins, Up-Regulation, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Cytokine Receptor gp130, Receptor-Like Protein Tyrosine Phosphatases, Class 7
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