TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis
Open accessAbstract
Background: According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We tested for the first time the hypothesis that EM TRIzol extraction allows proteomic Surface Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry (SELDI-TOF MS) analysis and that these proteomic data can be related to mRNA (microarray) data obtained from the same EM sample from women with and without endometriosis. Methods: Proteomic analysis was performed using SELDI-TOF-MS of TRIzol-extracted EM obtained during secretory phase from patients without endometriosis (n = 6), patients with minimal-mild (n = 5) and with moderate-severe endometriosis (n = 5), classified according to the system of the American Society of Reproductive Medicine. Proteomic data were compared to mRNA microarray data obtained from the same EM samples. Results: In our SELDI-TOF MS study 32 peaks were differentially expressed in endometrium of all women with endometriosis (stages I-IV) compared with all controls during the secretory phase. Comparison of proteomic results with those from microarray revealed no corresponding genes/proteins. Conclusion: TRIzol treatment of secretory phase EM allows combined proteomic and mRNA microarray analysis of the same sample, but comparison between proteomic and microarray data was not evident, probably due to post-translational modifications.
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A larger study identified differential expression of 32 peptide peaks in secretory phase endometrium from 10 women with endometriosis (all rAFS stages) compared to that of 6 healthy women.
“A larger study identified differential expression of 32 peptide peaks in secretory phase endometrium from 10 women with endometriosis (all rAFS stages) compared to that of 6 healthy women [134].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [134]
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- Authors
- Fassbender, A., Simsa, P., Kyama, C. M., Waelkens, E., Mihalyi, A., Meuleman, C., Gevaert, O., Van de Plas, R., de Moor, B., & D'Hooghe, T. M.
- Affiliations
- Universitair Ziekenhuis Leuven (BE)
- KU Leuven (BE)
- Venue
- Reproductive Biology and Endocrinology
- Published
- 2010-10-21
- Volume
- 8
- Issue
- 1
- Pages
- 123-123
- ISSN
- 1477-7827 (ISSN-L)
- PMC
- PMC2987945
- OpenAlex
- W2168310612
- Cited by
- 23
- References
- 24
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 5 · 2012: 1
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Update notices
- erratum (2011-04-06)
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Reproductive Biology and Endocrinology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Fonds Wetenschappelijk Onderzoek
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Keywords
- endometriosis, eutopic endometrium, secretory phase endometrium, mRNA microarray, post-translational modification, endometriosis pathogenesis, endometrial gene expression
- MeSH terms
- Adult, Cell Fractionation, Endometriosis, Endometrium, Female, Guanidines, Humans, Luteal Phase, Phenols, RNA, Messenger, Specimen Handling, Uterine Diseases, Case-Control Studies, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteome, Proteomics, Microarray Analysis
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