The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria
Open accessAbstract
BACKGROUND: DNA damage in human spermatozoa is known to be associated with a variety of adverse clinical outcomes affecting both reproductive efficiency and the health and wellbeing of the offspring. However, the origin of this damage, its biochemical nature and strategies for its amelioration, still await resolution. METHODS: Using novel methods to simultaneously assess DNA fragmentation (modified TUNEL assay), DNA-base adduct formation (8-hydroxy-2'-deoxyguanosine [8OHdG]) and cell vitality, spermatozoa from a cohort of 50 assisted conception patients were examined and compared with a group of donors. Receiver operating characteristic (ROC) curve analysis was then used to examine the frequency distribution of the data and to determine optimized thresholds for identifying patients exhibiting abnormally high levels of DNA damage. RESULTS: 8OHdG formation and DNA fragmentation were highly correlated with each other and frequently associated with cell death. Percoll centrifugation improved sperm quality but, unexpectedly, increased 8OHdG formation in live cells, as did sperm fractionation using Puresperm gradients. ROC analysis indicated that the frequency distribution of 8OHdG and DNA fragmentation data were significantly different between patients and donors (P < 0.001), permitting the development of thresholds that would allow the accurate diagnosis of DNA damage in the male germ line. CONCLUSION: The aetiology of DNA damage in spermatozoa involves a cascade of changes that progress from the induction of oxidative stress and oxidized DNA base adduct formation to DNA fragmentation and cell death. Preparation of spermatozoa on discontinuous density gradients aggravates the problem by stimulating the formation of 8OHdG in live cells. However, the development of novel methods and optimized thresholds for diagnosing oxidative DNA damage in human spermatozoa should assist in the clinical management of this pathology.
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Citations
Cites
Oxidative stress and male infertility—a clinical perspective
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Generation of Reactive Oxygen Species, Lipid Peroxidation, and Human Sperm Function
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Aitken, R. J., Gordon, E., Harkiss, D., Twigg, J. P., Milne, P., Jennings, Z., & Irvine, D. S. (1998) · Biology of Reproduction · doi:10.1095/biolreprod59.5.1037 · Cited by 793
Self-citationJones, R., Mann, T., & Sherins, R. (1979) · Fertility and Sterility · doi:10.1016/s0015-0282(16)43999-3 · Cited by 791
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Self-citationKoppers, A. J., De Iuliis, G. N., Finnie, J. M., McLaughlin, E. A., & Aitken, R. J. (2008) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2007-2616 · Cited by 698
Self-citationLipid Peroxidation and Human Sperm Motility: Protective Role of Vitamin E
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Cited by
Gharagozloo, P., & Aitken, R. J. (2011) · Human Reproduction · doi:10.1093/humrep/der132 · Cited by 522
Self-citationCauses and consequences of oxidative stress in spermatozoa
Aitken, R. J., Gibb, Z., Baker, M. A., Drevet, J., & Gharagozloo, P. (2015) · Reproduction, Fertility and Development · doi:10.1071/rd15325 · Cited by 428
Self-citationConsistent age-dependent declines in human semen quality: A systematic review and meta-analysis
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Antioxidants for male subfertility
Smits, R. M., Mackenzie-Proctor, R., Yazdani, A., Stankiewicz, M. T., Jordan, V., & Showell, M. G. (2019) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd007411.pub4 · Cited by 277
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Metadata
- Authors
- Aitken, R. J., De Iuliis, G. N., Finnie, J. M., Hedges, A., & McLachlan, R. I.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-08-17
- Volume
- 25
- Issue
- 10
- Pages
- 2415-2426
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2142451890
- Cited by
- 395
- References
- 32
- FWCI
- 26.36
- Type
- journal article
- Language
- en
- Open access
- bronze
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