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Cited by 143Endometriosis Research and TreatmentMetabolomics and Mass Spectrometry StudiesOvarian function and disorders

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Discovery of phosphatidylcholines and sphingomyelins as biomarkers for ovarian endometriosis

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Vouk, K., Hevir, N., Ribič-Pucelj, M., Haarpaintner, G., Scherb, H., Osredkar, J., Möller, G., Prehn, C., Rižner, T. Lanišnik, Adamski, J.·2012·Human Reproduction
DOI 10.1093/humrep/des152
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Abstract

BACKGROUND: Current non-invasive diagnostic methods for endometriosis lack sensitivity and specificity. In search for new diagnostic biomarkers for ovarian endometriosis, we used a hypothesis-generating targeted metabolomics approach. METHODS: In a case-control study, we collected plasma of study participants and analysed their metabolic profiles. We selected a group of 40 patients with ovarian endometriosis who underwent laparoscopic surgery and a control group of 52 healthy women who underwent sterilization at the University Clinical Centre Ljubljana, Slovenia. Over 140 targeted analytes included glycerophospholipids, sphingolipids and acylcarnitines. The analytes were quantified by electrospray ionization tandem mass spectrometry. For assessing the strength of association between the metabolite or metabolite ratios and the disease, we used crude and adjusted odds ratios. A stepwise logistic regression procedure was used for selecting the best combination of biomarkers. RESULTS: Eight lipid metabolites were identified as endometriosis-associated biomarkers due to elevated levels in patients compared with controls. A model containing hydroxysphingomyelin SMOH C16:1 and the ratio between phosphatidylcholine PCaa C36:2 to ether-phospholipid PCae C34:2, adjusted for the effect of age and the BMI, resulted in a sensitivity of 90.0%, a specificity of 84.3% and a ratio of the positive likelihood ratio to the negative likelihood ratio of 48.3. CONCLUSIONS: Our results suggest that endometriosis is associated with elevated levels of sphingomyelins and phosphatidylcholines, which might contribute to the suppression of apoptosis and affect lipid-associated signalling pathways. Our findings suggest novel potential routes for therapy by specifically blocking highly up-regulated isoforms of phosphpolipase A2 and lysophosphatidylcholine acyltransferase 4.

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Authors
Vouk, K., Hevir, N., Ribič-Pucelj, M., Haarpaintner, G., Scherb, H., Osredkar, J., Möller, G., Prehn, C., Rižner, T. L., & Adamski, J.
Affiliations
  • University of Ljubljana (SI)
  • Ljubljana University Medical Centre (SI)
  • Helmholtz Munich (DE)
Corresponding author
Rižner, T. Lanišnik
ORCID
Venue
Human Reproduction
Publisher
Oxford University Press (OUP)
Published
2012-08-01
Volume
27
Issue
10
Pages
2955-2965
ISSN
  • 0268-1161 (ISSN-L)
  • 1460-2350
DOI
10.1093/humrep/des152
OpenAlex
W2129793603
Cited by
143
References
42
Citations by year
2026: 8 · 2025: 13 · 2024: 12 · 2023: 8 · 2022: 10 · 2021: 16 · 2020: 8 · 2019: 7 · 2018: 11 · 2017: 18 · 2016: 9 · 2015: 15 · 2014: 6 · 2013: 2
FWCI
8.57
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Human Reproduction (publishedVersion)
  • PubMed (publishedVersion)
  • Repository of the University of Ljubljana (University of Ljubljana) (publishedVersion, other-oa)
  • Site cant be reached (submittedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
Keywords
endometrioma, phosphatidylcholine, sphingomyelin, plasma lipid biomarkers, targeted metabolomics, non-invasive diagnosis, glycerophospholipids, sphingolipids, acylcarnitines, apoptosis, phospholipase A2
MeSH terms
Adult, Age Factors, Endometriosis, Female, Humans, Phosphatidylcholines, Sensitivity and Specificity, Sphingomyelins, Biomarkers, Body Mass Index, Likelihood Functions, Logistic Models, Case-Control Studies

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