Virucidal activity and viral protein modification by myeloperoxidase: A candidate for defense factor of human polymorphonuclear leukocytes against influenza virus infection
Abstract
Influenza virus (IFV) was inactivated by treatment with degranulated fluid (DF) or myeloperoxidase (MPO) from human polymorphonuclear leukocytes in the presence of H2O2. Hemagglutinin and neuraminidase activities of the virus were also reduced by DF treatment. In addition, treatment with DF or MPO in the presence of H2O2 resulted in delayed migration of viral proteins in SDS-PAGE, indicating extensively modified viral proteins. Parallel with this aberrant migration, the radioactivity of each protein band on SDS-PAGE using [35S]methionine-labeled IFV was greatly reduced. Moreover, some of the modified viral protein did not migrate. A small amount of acid-soluble degraded viral peptide was also generated. The modification of the proteins was exhibited in all major viral proteins, including the inner proteins in the envelope. These results suggest that inactivation of IFV and protein modification by DF is due to MPO present in DF.
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Influenza virus was inactivated by MPO treatment of human polymorphonuclear leukocytes in the presence of H2O2, and viral protein modification occurred in all major proteins, including inner envelope proteins.
“On the treatment of viral infections, Yamamoto et al., 1991, show already at that time that the influenza virus IFV was inactivated by MPO-myeloperoxidase treatment of human polymorphonuclear leukocytes in the presence of H2O2 and that viral protein modification occurred in all major proteins, including the inner envelope proteins [161].”
From The Role of Reactive Species on Innate Immunity (2022) · cites this paper as [161]
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The Role of Reactive Species on Innate Immunity
Andrés, C., Pérez de la Lastra, J., Juan, C., Plou, F., & Pérez-Lebeña, E. (2022) · Vaccines · doi:10.3390/vaccines10101735 · Cited by 142 · see citing works
- Authors
- Yamamoto, K., Miyoshi-Koshio, T., Utsuki, Y., Mizuno, S., & Suzuki, K.
- ORCID
- Venue
- Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 1991-07-01
- Volume
- 164
- Issue
- 1
- Pages
- 8-14
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W1978686793
- Cited by
- 65
- References
- 34
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 2
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- myeloperoxidase, influenza virus, virucidal activity, polymorphonuclear leukocytes, hydrogen peroxide, hemagglutinin, neuraminidase, innate immunity, virus inactivation
- MeSH terms
- Cells, Cultured, Densitometry, Electrophoresis, Polyacrylamide Gel, Hemagglutinins, Viral, Humans, Peroxidase, Neuraminidase, Neutrophils, Influenza A virus, Viral Matrix Proteins, Viral Proteins, Cell Degranulation
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