Cited by 65Neutrophil, Myeloperoxidase and Oxidative MechanismsInfluenza Virus Research StudiesVirology and Viral Diseases

Virucidal activity and viral protein modification by myeloperoxidase: A candidate for defense factor of human polymorphonuclear leukocytes against influenza virus infection

Yamamoto, K., Miyoshi-Koshio, T., Utsuki, Y., Mizuno, S., Suzuki, K.·1991·Journal of Infectious Diseases

Abstract

Influenza virus (IFV) was inactivated by treatment with degranulated fluid (DF) or myeloperoxidase (MPO) from human polymorphonuclear leukocytes in the presence of H2O2. Hemagglutinin and neuraminidase activities of the virus were also reduced by DF treatment. In addition, treatment with DF or MPO in the presence of H2O2 resulted in delayed migration of viral proteins in SDS-PAGE, indicating extensively modified viral proteins. Parallel with this aberrant migration, the radioactivity of each protein band on SDS-PAGE using [35S]methionine-labeled IFV was greatly reduced. Moreover, some of the modified viral protein did not migrate. A small amount of acid-soluble degraded viral peptide was also generated. The modification of the proteins was exhibited in all major viral proteins, including the inner proteins in the envelope. These results suggest that inactivation of IFV and protein modification by DF is due to MPO present in DF.

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Crossref · 10.1093/infdis/164.1.8

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  • Influenza virus was inactivated by MPO treatment of human polymorphonuclear leukocytes in the presence of H2O2, and viral protein modification occurred in all major proteins, including inner envelope proteins.

    “On the treatment of viral infections, Yamamoto et al., 1991, show already at that time that the influenza virus IFV was inactivated by MPO-myeloperoxidase treatment of human polymorphonuclear leukocytes in the presence of H2O2 and that viral protein modification occurred in all major proteins, including the inner envelope proteins [161].”

    From The Role of Reactive Species on Innate Immunity (2022) · cites this paper as [161]

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Authors
Yamamoto, K., Miyoshi-Koshio, T., Utsuki, Y., Mizuno, S., & Suzuki, K.
Venue
Journal of Infectious Diseases
Published
1991-07-01
Volume
164
Issue
1
Pages
8-14
ISSN
  • 0022-1899 (ISSN-L)
  • 1537-6613
OpenAlex
W1978686793
Cited by
65
References
34
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 2
FWCI
0.69
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Infectious Diseases (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
myeloperoxidase, influenza virus, virucidal activity, polymorphonuclear leukocytes, hydrogen peroxide, hemagglutinin, neuraminidase, innate immunity, virus inactivation
MeSH terms
Cells, Cultured, Densitometry, Electrophoresis, Polyacrylamide Gel, Hemagglutinins, Viral, Humans, Peroxidase, Neuraminidase, Neutrophils, Influenza A virus, Viral Matrix Proteins, Viral Proteins, Cell Degranulation

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