Cited by 68Tuberculosis Research and EpidemiologyImmune responses and vaccinationsImmune Response and Inflammation

Interleukin 22 Inhibits Intracellular Growth of Mycobacterium tuberculosis by Enhancing Calgranulin A Expression

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Dhiman, Rohan, Venkatasubramanian, Sambasivan, Paidipally, Padmaja, Barnes, Peter F., Tvinnereim, Amy, Vankayalapati, Ramakrishna·2014·The Journal of Infectious Diseases

Abstract

Previously, we found that interleukin 22 (IL-22) inhibits intracellular growth of Mycobacterium tuberculosis in human monocyte-derived macrophages (MDMs). In the current study, we determined the mechanisms underlying these effects. We found that W7, a phagolysosomal fusion inhibitor, abrogates IL-22-dependent M. tuberculosis growth inhibition in MDMs, suggesting that IL-22 acts through enhanced phagolysosomal fusion. Our microarray analysis indicated that recombinant IL-22 (rIL-22) enhances the expression of an intracellular signaling molecule, calgranulin A. This was confirmed by real-time polymerase chain reaction, Western blot, and confocal microscopy. Calgranulin A small interfering RNA (siRNA) abrogated rIL-22-dependent growth inhibition of M. tuberculosis in MDMs. IL-22 enhanced Rab7 expression and downregulated Rab14 expression of M. tuberculosis-infected MDMs, and these effects were reversed by calgranulin A siRNA. These results suggest that M. tuberculosis growth inhibition by IL-22 depends on calgranulin A and enhanced phagolysosomal fusion, which is associated with increased Rab7 and reduced Rab14 expression.

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  • IL-22 exerts direct anti-mycobacterial activity in macrophages by stimulating phagolysosomal fusion.

    “whereas another Th17-derived cytokine, IL-22, exerts direct anti-mycobacterial activity in macrophages by stimulating phagolysosomal fusion.”

    From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [106]

  • The effect of IL-22 on phagolysosomal fusion is traced to IL-22-mediated activation of calgranulin A with subsequent induction of Rab7 and inhibition of Rab14 expression.

    “This effect could be traced back to IL-22-mediated activation of calgranulin A with subsequent induction of Rab7 and inhibition of Rab14 expression (106).”

    From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [106]

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Research works printed but not linked: 1

  1. Wolk K, Witte E, Witte K, Warszawska K, Sabat R. Biology of interleu- kin-22. Semin Immunopathol 2010; 32:17-31.

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Authors
Dhiman, R., Venkatasubramanian, S., Paidipally, P., Barnes, P. F., Tvinnereim, A., & Vankayalapati, R.
Affiliations
The University of Texas Health Science Center at Tyler (US)
Corresponding author
Dhiman, Rohan
Venue
The Journal of Infectious Diseases
Published
2013-09-16
Volume
209
Issue
4
Pages
578-587
ISSN
  • 0022-1899 (ISSN-L)
  • 1537-6613
OpenAlex
W2111494418
Cited by
68
References
50
Citations by year
2026: 3 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 9 · 2020: 8 · 2019: 3 · 2018: 11 · 2017: 8 · 2016: 4 · 2015: 8 · 2014: 1 · 2013: 1
FWCI
3.40
Type
journal article
Language
en
Open access
Locations
  • The Journal of Infectious Diseases (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Public Health Service (A1085135)
  • National Institute of Allergy and Infectious Diseases (R01AI054629, AI054629, AI06366)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
IL-22, Mycobacterium tuberculosis, S100A8, phagolysosomal fusion, monocyte-derived macrophages, Rab7, Rab14
MeSH terms
rab7 GTP-Binding Proteins, Interleukin-22, Cells, Cultured, Humans, Interleukins, Macrophages, Mycobacterium tuberculosis, Phagocytosis, Phagosomes, Recombinant Proteins, Statistics, Nonparametric, Oligonucleotide Array Sequence Analysis, rab GTP-Binding Proteins, RNA, Small Interfering, Calgranulin A, Gene Knockdown Techniques

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