Significant Correlation Between the Infant Gut Microbiome and Rotavirus Vaccine Response in Rural Ghana
Open accessAbstract
BACKGROUND: Rotavirus (RV) is the leading cause of diarrhea-related death in children worldwide and 95% of RV-associated deaths occur in Africa and Asia where RV vaccines (RVVs) have lower efficacy. We hypothesize that differences in intestinal microbiome composition correlate with the decreased RVV efficacy observed in poor settings. METHODS: We conducted a nested, case-control study comparing prevaccination, fecal microbiome compositions between 6-week old, matched RVV responders and nonresponders in rural Ghana. These infants' microbiomes were then compared with 154 age-matched, healthy Dutch infants' microbiomes, assumed to be RVV responders. Fecal microbiome analysis was performed in all groups using the Human Intestinal Tract Chip. RESULTS: We analyzed findings in 78 Ghanaian infants, including 39 RVV responder and nonresponder pairs. The overall microbiome composition was significantly different between RVV responders and nonresponders (FDR, 0.12), and Ghanaian responders were more similar to Dutch infants than nonresponders (P = .002). RVV response correlated with an increased abundance of Streptococcus bovis and a decreased abundance of the Bacteroidetes phylum in comparisons between both Ghanaian RVV responders and nonresponders (FDR, 0.008 vs 0.003) and Dutch infants and Ghanaian nonresponders (FDR, 0.002 vs 0.009). CONCLUSIONS: The intestinal microbiome composition correlates significantly with RVV immunogenicity and may contribute to the diminished RVV immunogenicity observed in developing countries.
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In 78 Ghanaian infants, overall microbiome composition was significantly different between responders and non-responders to Rotarix vaccination, with Ghanaian responders being more similar to healthy Dutch infants than non-responders.
“The overall microbiome composition was significantly different between responders and non-responders to vaccination, with the Ghanaian responders being more similar to healthy Dutch infants than non-responders.”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Harris et al., 2017
Responses to Rotarix correlated with an increased abundance of Streptococcus bovis, and lack of response correlated with high abundance of Bacteroidetes, especially Bacteroides and Prevotella.
“Responses to Rotarix® correlated with an increased abundance of Streptococcus bovis and the lack of response is correlated with the high abundance of Bacteroidetes phylum, especially several bacteria related to species from the Bacteroides and Prevotella genera.”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Harris et al., 2017
Gut microbiome modulation by antibiotics modifies immune response to Rotavirus vaccination.
“The central role of the gut microbiome in viral immunity is highlighted in human vaccination studies in a dysbiosis context: gut microbiome modulation by antibiotics has been showed to modify immune response to Rotavirus vaccination (Harris et al., 2017).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Harris et al., 2017
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- Authors
- Harris, V. C., Armah, G., Fuentes, S., Korpela, K. E., Parashar, U., Victor, J. C., Tate, J., de Weerth, C., Giaquinto, C., Wiersinga, W. J., Lewis, K. D. C., & de Vos, W. M.
- Affiliations
- Amsterdam UMC Location University of Amsterdam (NL)
- University of Ghana (GH)
- Wageningen University & Research (NL)
- University of Helsinki (FI)
- Centers for Disease Control and Prevention (US)
- Program for Appropriate Technology in Health (US)
- Behavioral Science Institute, Radboud University, Nijmegen, The Netherlands
- University of Padua (IT)
- Laboratory of Microbiology, Wageningen University
- Corresponding author
- Harris, Vanessa C
- Lewis, Kristen D C
- ORCID
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-10-31
- Volume
- 215
- Issue
- 1
- Pages
- 34-41
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- PMC
- PMC5225256
- OpenAlex
- W2546024328
- Cited by
- 299
- References
- 39
- Citations by year
- 2026: 13 · 2025: 28 · 2024: 30 · 2023: 22 · 2022: 33 · 2021: 51 · 2020: 27 · 2019: 37 · 2018: 37 · 2017: 20 · 2016: 1
- FWCI
- 29.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The Journal of Infectious Diseases (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion, cc-by)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Työväentutkimus Vuosikirja (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Pure Amsterdam UMC (submittedVersion, cc-by)
- Radboud Repository (Radboud University) (submittedVersion)
- Padua Research Archive (University of Padova) (submittedVersion)
- Radboud Repository (Radboud University) (submittedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion, cc-by)
- Greater South Information System
- Funders
- Bill and Melinda Gates Foundation (OPP1017334)
- GlaxoSmithKline
- Sanofi
- Cincinnati Children's Hospital Medical Center
- Centers for Disease Control and Prevention
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- rotavirus vaccine, infant gut microbiota, vaccine response, vaccine immunogenicity, Streptococcus bovis, Bacteroidetes, fecal microbiota, rural Ghana, developing countries, childhood diarrhea, cross-population comparison
- MeSH terms
- Gastrointestinal Microbiome, Antibodies, Viral, Bacteria, Feces, Female, Gastroenteritis, Ghana, Humans, Immunoglobulin A, Infant, Male, Pregnancy, Rotavirus Infections, Rotavirus, Rural Population, Vaccines, Attenuated, Case-Control Studies, Streptococcus bovis, Immunity, Mucosal, Rotavirus Vaccines, Bacteroidetes, Microarray Analysis
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