Cited by 4,129Bacterial Identification and Susceptibility TestingAntibiotic Resistance in BacteriaAntimicrobial Resistance in Staphylococcus

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Determination of minimum inhibitory concentrations

Andrews, Jennifer M.·2001·Journal of Antimicrobial Chemotherapy

DOI 10.1093/jac/48.suppl_1.5

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Abstract

Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation, and minimum bactericidal concentrations (MBCs) as the lowest concentration of antimicrobial that will prevent the growth of an organism after subculture on to antibiotic-free media. MICs are used by diagnostic laboratories mainly to confirm resistance, but most often as a research tool to determine the in vitro activity of new antimicrobials, and data from such studies have been used to determine MIC breakpoints. MBC determinations are undertaken less frequently and their major use has been reserved for isolates from the blood of patients with endocarditis. Standardized methods for determining MICs and MBCs are described in this paper. Like all standardized procedures, the method must be adhered to and may not be adapted by the user. The method gives information on the storage of standard antibiotic powder, preparation of stock antibiotic solutions, media, preparation of inocula, incubation conditions, and reading and interpretation of results. Tables giving expected MIC ranges for control NCTC and ATCC strains are also supplied.

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Report of the Working Party on Antibiotic Sensitivity Testing of the British Society of Antimicrobial Chemotherapy. (1991). A guide to sensitivity testing. Journal of Antimicrobial Chemotherapy 27, Suppl. D, 1-50.

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Authors: Andrews, J. M.
Affiliations: Birmingham City Hospital (GB)
Corresponding author: Andrews, Jennifer M.
Venue: Journal of Antimicrobial Chemotherapy
Publisher: Oxford University Press (OUP)
Published: 2001-07-01
Volume: 48
Issue: suppl_1
Pages: 5-16
ISSN: 0305-7453 (ISSN-L)
1460-2091
DOI: 10.1093/jac/48.suppl_1.5
OpenAlex: W2132963724
Cited by: 4,129
References: 4
Citations by year: 2026: 177 · 2025: 264 · 2024: 253 · 2023: 265 · 2022: 311 · 2021: 287 · 2020: 309 · 2019: 255 · 2018: 232 · 2017: 213 · 2016: 238 · 2015: 234 · 2014: 217 · 2013: 209 · 2012: 144
FWCI: 9.12
Type: journal article
Language: en
Open access: closed
Locations: Journal of Antimicrobial Chemotherapy (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
Keywords: minimum inhibitory concentration, minimum bactericidal concentration, antimicrobial susceptibility testing, standardized test methods, antimicrobial resistance, MIC breakpoints, control strains
MeSH terms: Anti-Bacterial Agents, Bacteria, Microbial Sensitivity Tests

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Determination of minimum inhibitory concentrations

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