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Determination of minimum inhibitory concentrations
Abstract
Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation, and minimum bactericidal concentrations (MBCs) as the lowest concentration of antimicrobial that will prevent the growth of an organism after subculture on to antibiotic-free media. MICs are used by diagnostic laboratories mainly to confirm resistance, but most often as a research tool to determine the in vitro activity of new antimicrobials, and data from such studies have been used to determine MIC breakpoints. MBC determinations are undertaken less frequently and their major use has been reserved for isolates from the blood of patients with endocarditis. Standardized methods for determining MICs and MBCs are described in this paper. Like all standardized procedures, the method must be adhered to and may not be adapted by the user. The method gives information on the storage of standard antibiotic powder, preparation of stock antibiotic solutions, media, preparation of inocula, incubation conditions, and reading and interpretation of results. Tables giving expected MIC ranges for control NCTC and ATCC strains are also supplied.
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“This difference is important to consider when performing experiments that depend on the number of cellular divisions or cells present, such as cellular communication [12, 13] and antibiotic susceptibility [14–17] and biofilms [18].”
From Estimating microbial population data from optical density (2022) · cites this paper as [14]
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Metadata
- Authors
- Andrews, J. M.
- Affiliations
- Birmingham City Hospital (GB)
- Corresponding author
- Andrews, Jennifer M.
- Venue
- Journal of Antimicrobial Chemotherapy
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-07-01
- Volume
- 48
- Issue
- suppl_1
- Pages
- 5-16
- ISSN
- 0305-7453 (ISSN-L)
- 1460-2091
- OpenAlex
- W2132963724
- Cited by
- 4,129
- References
- 4
- Citations by year
- 2026: 177 · 2025: 264 · 2024: 253 · 2023: 265 · 2022: 311 · 2021: 287 · 2020: 309 · 2019: 255 · 2018: 232 · 2017: 213 · 2016: 238 · 2015: 234 · 2014: 217 · 2013: 209 · 2012: 144
- FWCI
- 9.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Antimicrobial Chemotherapy (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- minimum inhibitory concentration, minimum bactericidal concentration, antimicrobial susceptibility testing, standardized test methods, antimicrobial resistance, MIC breakpoints, control strains
- MeSH terms
- Anti-Bacterial Agents, Bacteria, Microbial Sensitivity Tests
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