The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures
Abstract
OBJECTIVES: Effective disinfection and antisepsis is pivotal in preventing infections within the healthcare setting. Chlorhexidine digluconate (CHG) is a widely used disinfectant/antiseptic possessing broad-spectrum antimicrobial activity; however, its penetration into bacterial biofilms and human skin is poor. The aim of this study was to investigate the antimicrobial efficacy of crude eucalyptus oil (EO) and its main component 1,8-cineole (a recognized permeation enhancer), alone and in combination with CHG, against a panel of clinically relevant microorganisms grown in planktonic and biofilm cultures. METHODS: MICs and minimum bactericidal/fungicidal concentrations were determined for each microorganism grown in suspension and biofilm using microbroth dilution and ATP bioluminescence, respectively. Chequerboard assays were used to determine synergistic, indifferent or antagonistic interactions between CHG and EO or 1,8-cineole. RESULTS: Antimicrobial activity was demonstrated by CHG, EO and 1,8-cineole; however, CHG was significantly more active against microorganisms in both planktonic and biofilm modes of growth (P < 0.05). Crude EO was significantly more efficacious against microorganisms grown in suspension compared with 1,8-cineole (P < 0.05). Synergistic activity was demonstrated between CHG and both EO and 1,8-cineole against suspensions of Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Escherichia coli and Candida albicans, and biofilm cultures of MRSA and Pseudomonas aeruginosa. CONCLUSIONS: In conclusion, CHG may be combined with either crude EO or its major component 1,8-cineole for enhanced, synergistic antimicrobial activity against a wide range of microorganisms in planktonic and biofilm modes of growth; however, the superior antimicrobial efficacy associated with crude EO alone, compared with 1,8-cineole, favours its combination with CHG.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Plant Products as Antimicrobial Agents
Cowan, M. M. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.4.564 · Cited by 9,043
Antimicrobial activity of essential oils and other plant extracts
Hammer, K. A., Carson, C. F., & Riley, T. V. (1999) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1999.00780.x · Cited by 2,560
Cited by
Why do plants produce so many terpenoid compounds?
Pichersky, E., & Raguso, R. A. (2018) · New Phytologist · doi:10.1111/nph.14178 · Cited by 709
van Vuuren, S., & Viljoen, A. (2011) · Planta Medica · doi:10.1055/s-0030-1250736 · Cited by 367
1,8-Cineole: a review of source, biological activities, and application
Cai, Z.-M., Peng, J.-Q., Chen, Y., Tao, L., Zhang, Y.-Y., Fu, L.-Y., Long, Q.-D., & Shen, X.-C. (2021) · Journal of Asian Natural Products Research · doi:10.1080/10286020.2020.1839432 · Cited by 308
Kouidhi, B., Al Qurashi, Y. M. A., & Chaieb, K. (2015) · Microbial Pathogenesis · doi:10.1016/j.micpath.2015.02.007 · Cited by 180
Ameur, E., Sarra, M., Yosra, D., Mariem, K., Nabil, A., Lynen, F., & Larbi, K. M. (2021) · BMC Complementary Medicine and Therapies · doi:10.1186/s12906-021-03379-y · Cited by 36
Metadata
- Authors
- Hendry, E. R., Worthington, T., Conway, B. R., & Lambert, P. A.
- Venue
- Journal of Antimicrobial Chemotherapy
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-10-16
- Volume
- 64
- Issue
- 6
- Pages
- 1219-1225
- ISSN
- 0305-7453 (ISSN-L)
- 1460-2091
- OpenAlex
- W2160861478
- Cited by
- 291
- References
- 2
- FWCI
- 12.18
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures. https://app.cochat.ai/papers/10.1093/jac/dkp362
- The paper
- Hendry, E. R., Worthington, T., Conway, B. R., & Lambert, P. A. (2009). Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures. Journal of Antimicrobial Chemotherapy. https://doi.org/10.1093/jac/dkp362
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
