Identification of avian and hemoparasite DNA in blood-engorged abdomens of Culex pipiens (Diptera; Culicidae) from a west nile virus epidemic region in suburban Chicago, Illinois
Abstract
Multiple mosquito-borne parasites cocirculate in nature and potentially interact. To understand the community of parasites cocirculating with West Nile virus (WNV), we screened the bloodmeal content of Culex pipiens L. mosquitoes for three common types of hemoparasites. Blood-fed Cx. pipiens were collected from a WNV-epidemic area in suburban Chicago, IL, from May to September 2005 through 2010. DNA was extracted from dissected abdomens and subject to PCR and direct sequencing to identify the vertebrate host. RNA was extracted from the head or thorax and screened for WNV using quantitative reverse transcriptase PCR. Seventy-nine engorged females with avian host origin were screened using PCR and amplicon sequencing for filarioid nematodes, Haemosporida, and trypanosomatids. Filarioid nematodes were identified in 3.8% of the blooded abdomens, Plasmodium sp. in 8.9%, Haemoproteus in 31.6%, and Trypanosoma sp. in 6.3%. The sequences from these hemoparasite lineages were highly similar to sequences from birds in prior studies in suburban Chicago. Overall, 50.6% of blood-fed Culex pipiens contained hemoparasite DNA in their abdomen, presumably from current or prior bloodmeals. Additionally, we detected hemoparasite DNA in the blooded abdomen of three of 10 Cx. pipiens infected with WNV.
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Boothe et al. (2015) reported detection of filarioid nematodes in 3.8% of blood-fed Cx. pipiens.
“while Boothe et al. (2015) reported detection of filarioid nematodes in 3.8% of blood-fed Cx. pipiens.”
From Culex pipiens as a potential vector for transmission of Dirofilaria immitis and other unclassified Filarioidea in Southwest Spain (2016) · cites this paper as Boothe et al. (2015)
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- Authors
- Boothe, E., Medeiros, M. C. I., Kitron, U. D., Brawn, J. D., Ruiz, M. O., Goldberg, T. L., Walker, E. D., & Hamer, G. L.
- Affiliations
- Texas A&M University (US)
- University of Missouri–St. Louis (US)
- Emory University (US)
- University of Illinois Urbana-Champaign (US)
- University of Wisconsin–Madison (US)
- Michigan State University (US)
- Venue
- Journal of Medical Entomology
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-03-31
- Volume
- 52
- Issue
- 3
- Pages
- 461-468
- ISSN
- 0022-2585 (ISSN-L)
- 1938-2928
- OpenAlex
- W2006382755
- Cited by
- 13
- References
- 52
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medical Entomology (publishedVersion)
- PubMed (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Diptera species taxonomy and behavior
- Keywords
- Culex pipiens, West Nile virus, filarioid nematodes, Haemoproteus, Plasmodium, trypanosomes, mosquito-borne parasites, suburban Chicago
- MeSH terms
- Animals, Culex, DNA, Filarioidea, Illinois, Molecular Sequence Data, Phylogeny, Columbidae, Trypanosomatina, West Nile Fever, DNA, Protozoan, Haemosporida, Sequence Analysis, DNA, DNA, Helminth, Songbirds
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