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In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors2
Abstract
A series of 200 human tumors were cultivated in vitro in an attempt to establish cell lines. Lines were established, with explant and trypsinization techniques, from 13 tumors including carcinomas, sarcomas, melanomas, and brain tumors. All these lines, In culture for over 1 year, exhibited marked refractility, multilayering, and criss-crossing and were morphologically distinct from normal contact-inhibited human fibroblast or epithelial lines. They also formed colonies on monolayers of normal cells and grew with a high efficiency in soft agar. Preliminary results indicated abnormal chromosomal patterns in all lines tested, and 8 of 9 cell lines formed tumors in antithymo~yte serum-treated mice. The rate of establishment (approximately 6%) of lines from random neoplastic material demonstrated that cells with properties of transformed cells could be recovered from tumor tissue, but it also emphasized the need for improved methodology in this area.
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Citations
Cited by
Yoshida, M., Miyoshi, I., & Hinuma, Y. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.6.2031 · Cited by 2,169
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Michelucci, A., Cordes, T., Ghelfi, J., Pailot, A., Reiling, N., Goldmann, O., Binz, T., Wegner, A., Tallam, A., Rausell, A., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1218599110 · Cited by 1,191
Export of microRNAs and microRNA-protective protein by mammalian cells
Wang, K., Zhang, S., Weber, J., Baxter, D., & Galas, D. J. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq601 · Cited by 1,002
Oops they did it again! Carbon nanotubes hoax scientists in viability assays
Wörle-Knirsch, J. M., Pulskamp, K., & Krug, H. F. (2006) · Nano Letters · doi:10.1021/nl060177c · Cited by 994
Isolation of a human prostate carcinoma cell line (DU 145)
Stone, K. R., Mickey, D. D., Wunderli, H., Mickey, G. H., & Paulson, D. F. (1978) · International Journal of Cancer · doi:10.1002/ijc.2910210305 · Cited by 926
Metadata
- Authors
- Giard, D. J., Aaronson, S. A., Todaro, G. J., Arnstein, P., Kersey, J. H., Dosik, H., & Parks, W. P.
- Venue
- JNCI: Journal of the National Cancer Institute
- Publisher
- Oxford University Press (OUP)
- Published
- 1973-11
- Volume
- 51
- Issue
- 5
- Pages
- 1417-1423
- ISSN
- 0027-8874 (ISSN-L)
- 1460-2105
- OpenAlex
- W2102838769
- Cited by
- 2,289
- References
- 0
- FWCI
- 13.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors2. https://app.cochat.ai/papers/10.1093/jnci/51.5.1417
- The paper
- Giard, D. J., Aaronson, S. A., Todaro, G. J., Arnstein, P., Kersey, J. H., Dosik, H., & Parks, W. P. (1973). In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors2. JNCI: Journal of the National Cancer Institute. https://doi.org/10.1093/jnci/51.5.1417
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
