The DOI matches its Crossref record; 88 of the 88 references it cites were found in a registry or an index.
Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum
Open accessAbstract
Algal growth is strongly affected by nitrogen (N) availability. Diatoms, an ecologically important group of unicellular algae, have evolved several acclimation mechanisms to cope with N deprivation. In this study, we integrated physiological data with transcriptional and metabolite data to reveal molecular and metabolic modifications in N-deprived conditions in the marine diatom Phaeodactylum tricornutum. Physiological and metabolite measurements indicated that the photosynthetic capacity and chlorophyll content of the cells decreased, while neutral lipids increased in N-deprived cultures. Global gene expression analysis showed that P. tricornutum responded to N deprivation through an increase in N transport, assimilation, and utilization of organic N resources. Following N deprivation, reduced biosynthesis and increased recycling of N compounds like amino acids, proteins, and nucleic acids was observed at the transcript level. The majority of the genes associated with photosynthesis and chlorophyll biosynthesis were also repressed. Carbon metabolism was restructured through downregulation of the Calvin cycle and chrysolaminarin biosynthesis, and co-ordinated upregulation of glycolysis, the tricarboxylic acid cycle, and pyruvate metabolism, leading to funnelling of carbon sources to lipid metabolism. Finally, reallocation of membrane lipids and induction of de novo triacylglycerol biosynthesis directed cells to accumulation of neutral lipids.
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Cited by
Kumar, G., Shekh, A., Jakhu, S., Sharma, Y., Kapoor, R., & Sharma, T. R. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00914 · Cited by 315
Current trends to comprehend lipid metabolism in diatoms
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Zienkiewicz, K., Du, Z.-Y., Ma, W., Vollheyde, K., & Benning, C. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2016.02.008 · Cited by 225
Latest Trends and Developments in Microalgae as Potential Source for Biofuels: The Case of Diatoms
Mourya, M., Khan, M. J., Ahirwar, A., Schoefs, B., Marchand, J., Rai, A., Varjani, S., Rajendran, K., Banu, J. R., & Vinayak, V. (2022) · Fuel · doi:10.1016/j.fuel.2021.122738 · Cited by 52
Maltsev, Y., Maltseva, I., Maltseva, S., Kociolek, J. P., & Kulikovskiy, M. (2021) · Journal of Phycology · doi:10.1111/jpy.13116 · Cited by 31
Metadata
- Authors
- Alipanah, L., Rohloff, J., Winge, P., Bones, A. M., & Brembu, T.
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-07-10
- Volume
- 66
- Issue
- 20
- Pages
- 6281-6296
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W2099505680
- Cited by
- 278
- References
- 88
- FWCI
- 5.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Alipanah, L., Rohloff, J., Winge, P., Bones, A. M., & Brembu, T. (2015). Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum. Journal of Experimental Botany. https://doi.org/10.1093/jxb/erv340
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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