Cited by 139Reproductive System and PregnancyEstrogen and related hormone effectsReproductive Biology and Fertility

Regulation of HOXA-10 and its expression in normal and abnormal endometrium

Gui, Yaoting, Zhang, Jenning, Yuan, Lingwen, Lessey, Bruce A.·1999·MHR: Basic science of reproductive medicine

Abstract

HOXA-10 is a member of a family of genes that serve as transcription factors during development and have been shown to be important for uterine function. Using immunohistochemistry and RNAse protection assays (RPA), HOXA-10 was shown to be expressed in both epithelial and stromal cells with increased expression during the window of implantation. By in-vitro culture of isolated endometrial epithelium or stroma, HOXA-10, expression was increased after treatment with oestradiol (10(-8) mol/l) with or without progesterone (10(-6) mol/l). In stromal cells, oestradiol and progesterone both appeared to increase HOXA-10 expression and were additive. Relaxin (30 ng/ml) appeared to further increase stromal HOXA-10 expression. HOXA-10 expression during the window of implantation was compared in normal menstrual cycles to endometrium from women with endometriosis and suspected defects in uterine receptivity. Little or no difference was seen in luminal, glandular or endothelial HOXA-10 expression but a significant reduction in stroma HOXA-10 expression was noted in women with endometriosis. In conclusion, HOXA-10 is a hormone-regulated endometrial transcription factor that appears to be responsive to both ovarian steroids and relaxin. The appearance of this nuclear protein during the window of implantation in epithelium and stroma may offer new insight into the regulation of uterine receptivity and assist in the identification of other genes that are critical to the establishment of a successful pregnancy.

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Crossref · 10.1093/molehr/5.9.866

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  • The endometrium of women with endometriosis responds differently to ovarian hormones.

    “It is important to emphasize that the endometrium of women with endometriosis has been documented to respond differently to ovarian hormones (83-86), and we can use this fact to discover new potential biomarkers through minimally invasive means (87).”

    From Biomarkers in endometriosis: Challenges and opportunities (2017) · cites this paper as [83]

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Authors
Gui, Y., Zhang, J., Yuan, L., & Lessey, B. A.
Affiliations
University of North Carolina at Chapel Hill (US)
Venue
MHR: Basic science of reproductive medicine
Published
1999-09
Volume
5
Issue
9
Pages
866-873
ISSN
  • 1360-9947 (ISSN-L)
  • 1460-2407
OpenAlex
W2144684054
Cited by
139
References
43
Citations by year
2026: 1 · 2025: 5 · 2024: 2 · 2023: 7 · 2022: 7 · 2021: 4 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 2 · 2015: 4 · 2014: 8 · 2013: 2 · 2012: 5
FWCI
1.60
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Human Reproduction (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5u01hd034824-04)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-34824, U54HD035041, HD35041)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
Keywords
HOXA10, endometrium, endometriosis, endometrial receptivity, implantation window, estradiol, progesterone, relaxin, endometrial stroma, endometrial epithelium, transcription factors, pregnancy establishment
MeSH terms
Fulvestrant, Homeobox A10 Proteins, Cells, Cultured, DNA-Binding Proteins, Dose-Response Relationship, Drug, Endometriosis, Endometrium, Estradiol, Estrogen Antagonists, Female, Hormone Antagonists, Humans, Menstrual Cycle, Embryo Implantation, Progesterone, Reference Values, Relaxin, Mifepristone, Stromal Cells, Homeodomain Proteins

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