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Cited by 70Evolution and Genetic DynamicsDNA Repair MechanismsBacterial Genetics and Biotechnology

Divergent adaptation of Escherichia coli to cyclic ultraviolet light exposures

Alcantara-Diaz, D.·2004·Mutagenesis
DOI 10.1093/mutage/geh039
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Abstract

The genetic changes taking place during adaptive evolution are particularly interesting in evolutionary biology. As a consequence of adaptive evolution, natural populations of an organism under selective conditions change genetically and phenotypically after a number of generations in order to survive in that particular environment. When a DNA-damaging and mutagenic agent like UV light is experimentally used as a selective factor, natural resistance of bacteria to this agent is normally increased through processes of mutation and selection. Since UV-induced mutagenesis is not restricted to particular chromosomal regions, different UV resistance mechanisms will equally probably evolve as a consequence of cyclic UV irradiation. However, it is also possible that as a consequence of the selective process, one UV resistance mechanism is preferentially selected, causing adaptive convergence of different bacterial cultures. This may occur if the most abundant or lethal kind of DNA lesion is preferentially managed by a particular DNA repair pathway and even by a specific repair enzyme or if resistance mechanisms that decrease bacterial fitness tend to be eliminated from the populations. To examine which of these two alternatives actually takes place, five cultures of Escherichia coli were treated in parallel for 80 successive UV irradiation cycles. At the end, these five cultures gave rise to different grades of UV resistance and after a preliminary characterization we found that adaptation to cyclic UV irradiation was a consequence of selection of advantageous mutations arising in different genes related to repair and replication of DNA.

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  • In evolved ∆pgi E. coli strains, growth rates and glucose uptake rates increased up to 3.6 and up to 2.6 fold as compared with the ancestor, accompanied by different phenotypes regarding acetate and formate production.

    “Growth rates and glucose uptake rates increased up to 3.6 and up to 2.6 fold in evolved ∆pgi strains as compared with the ancestor and were accompanied with different phenotypes regarding acetate and formate production.”

    From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [70]

  • Evolved ∆pgi E. coli strains had frequent point mutations in rpoS, udhA, pntAB and a prophage deletion; these mutations were not observed in the glucose-adapted wildtype strain, while other regulatory mutations including rpoABC occurred in both backgrounds.

    “The authors found frequent point mutations in rpoS, udhA (soluble transhydrogenase), pntAB and a prophage deletion. They did not observe these mutations in the glucose-adapted wildtype strain; other regulatory mutations (including rpoABC) occurred in both backgrounds”

    From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [70]

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Authors
Alcantara-Diaz, D.
Venue
Mutagenesis
Publisher
Oxford University Press (OUP)
Published
2004-09-01
Volume
19
Issue
5
Pages
349-354
ISSN
  • 0267-8357 (ISSN-L)
  • 1464-3804
DOI
10.1093/mutage/geh039
OpenAlex
W2161040180
Cited by
70
References
38
Citations by year
2026: 4 · 2025: 5 · 2024: 6 · 2023: 3 · 2022: 3 · 2021: 10 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 3 · 2015: 4 · 2014: 6 · 2013: 4 · 2012: 3
FWCI
0.18
Type
journal article
Language
en
Open access
closed
Locations
  • Mutagenesis (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Escherichia coli, UV resistance, adaptive evolution, UV mutagenesis, DNA repair, adaptive convergence, DNA replication, bacterial fitness
MeSH terms
Alkaline Phosphatase, Chromosome Mapping, DNA Damage, DNA Repair, DNA Replication, Dose-Response Relationship, Radiation, Escherichia coli, Genetic Techniques, Light, Mutagens, Mutation, Radiation Tolerance, Time Factors, Ultraviolet Rays

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