Microbial evolution in vivo and in silico: methods and applications
Abstract
Microbial evolution has been extensively studied in the past fifty years, which has lead to seminal discoveries that have shaped our understanding of evolutionary forces and dynamics. It is only recently however, that transformative technologies and computational advances have enabled a larger in-scale and in-depth investigation of the genetic basis and mechanistic underpinnings of evolutionary adaptation. In this review we focus on the strengths and limitations of in vivo and in silico techniques for studying microbial evolution in the laboratory, and we discuss how these complementary approaches can be integrated in a unifying framework for elucidating microbial evolution.
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During adaptive evolution, certain trait values change and are associated with increased Darwinian fitness; an improved phenotype or property is often equal to increased fitness.
“During adaptive evolution, certain trait values change and are associated with increased (Darwinian) fitness [20]; as such, an improved phenotype or property is often equal to increased fitness.”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [20]
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- Authors
- Mozhayskiy, V., & Tagkopoulos, I.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- Tagkopoulos, Ilias
- ORCID
- Tagkopoulos, Ilias
- Venue
- Integr. Biol.
- Publisher
- Oxford University Press (OUP)
- Published
- 2013
- Volume
- 5
- Issue
- 2
- Pages
- 262-277
- ISSN
- 1757-9694 (ISSN-L)
- 1757-9708
- OpenAlex
- W2144526734
- Cited by
- 24
- References
- 140
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2
- FWCI
- 0.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Integrative Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of California, Davis
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- microbial evolution, in vivo evolution, in silico evolution, experimental evolution, adaptive evolution, genetic basis of adaptation
- MeSH terms
- Bacteria, Bacterial Proteins, Computer Simulation, Biological Evolution, Models, Genetic, Mutation
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