The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Polymer support oligonucleotide synthesis XVIII1.2): use of β-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product
Open accessAbstract
Various 5'O-N-protected deoxynucleoside-3'-O-beta-cyanoethyl-N,N-dialkylamino-/N- morpholinophosphoramidites were prepared from beta-cyanoethyl monochlorophosphoramidites of N,N-dimethylamine, N,N-diisopropylamine and N-morpholine. These active deoxynucleoside phosphates have successfully been used for oligodeoxynucleotide synthesis on controlled pore glass as polymer support and are very suitable for automated DNA-synthesis due to their stability in solution. The intermediate dichloro-beta- cyanoethoxyphosphine can easily be prepared free from any PC1(3) contamination. The active monomers obtained from beta-cyanoethyl monochloro N,N- diisopropylaminophosphoramidites are favoured. Cleavage of the oligonucleotide chain from the polymer support, N-deacylation and deprotection of beta-cyanoethyl group from the phosphate triester moiety can be performed in one step with concentrated aqueous ammonia. Mixed oligodeoxynucleotides are characterized by the sequencing method of Maxam and Gilbert.
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Cites
[57] Sequencing end-labeled DNA with base-specific chemical cleavages
Maxam, A. M., & Gilbert, W. (1980) · Methods in Enzymology · doi:10.1016/s0076-6879(80)65059-9 · Cited by 14,315
Synthesis of thymidine oligonucleotides by phosphite triester intermediates
Letsinger, R. L., & Lunsford, W. B. (1976) · Journal of the American Chemical Society · doi:10.1021/ja00428a045 · Cited by 283
Automated Synthesis of Gene Fragments
Alvarado-Urbina, G., Sathe, G. M., Liu, W.-C., Gillen, M. F., Duck, P. D., Bender, R., & Ogilvie, K. K. (1981) · Science · doi:10.1126/science.6169150 · Cited by 176
Nucleotide chemistry. XX. Phosphite coupling procedure for generating internucleotide links
Letsinger, R. L., Finnan, J. L., Heavner, G. A., & Lunsford, W. B. (1975) · Journal of the American Chemical Society · doi:10.1021/ja00844a090 · Cited by 157
Cited by
Carter, D. B., Deibel, M. R., Dunn, C. J., Tomich, C.-S. C., Laborde, A. L., Slightom, J. L., Berger, A. E., Bienkowski, M. J., Sun, F. F., McEwan, R. N., et al. (1990) · Nature · doi:10.1038/344633a0 · Cited by 601
Gallie, D. R., Sleat, D. E., Watts, J. W., Turner, P. C., & Wilson, T. M. A. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.8.3257 · Cited by 408
Takeshita, M., Chang, C. N., Johnson, F., Will, S., & Grollman, A. P. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61093-2 · Cited by 400
Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli.
Ikemura, H., Takagi, H., & Inouye, M. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47646-6 · Cited by 335
Metadata
- Authors
- Sinha, N. D., Biernat, J., McManus, J., & Köster, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1984
- Volume
- 12
- Issue
- 11
- Pages
- 4539-4557
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1984286262
- Cited by
- 849
- References
- 4
- FWCI
- 12.63
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Polymer support oligonucleotide synthesis XVIII1.2): use of β-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product. https://app.cochat.ai/papers/10.1093/nar/12.11.4539
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- Sinha, N. D., Biernat, J., McManus, J., & Köster, H. (1984). Polymer support oligonucleotide synthesis XVIII1.2): use of β-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product. Nucleic Acids Research. https://doi.org/10.1093/nar/12.11.4539
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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