The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
In vitro virus: Bonding of mRNA bearing puromycin at the 3′‐terminal end to the C‐terminal end of its encoded protein on the ribosome in vitro
Open accessAbstract
Adequate means for genotype assignment to phenotype is essential in evolutionary molecular engineering. In this study, construction of ‘in vitro virus’ was carried out in which a genotype molecule (mRNA) covalently binds to the phenotype molecule (protein) through puromycin on the ribosome in a cell‐free translation system. Bonding efficiency was ∼10%, thus indicating a population of the in vitro virus to have ∼10 12 protein variants, this number being 10 4 that in the phage display. The in vitro virus is useful for examining protein evolution in a test tube and the results may possibly serve as basis for a general method for selecting proteins possessing the most desirable functions.
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Cites
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In vitro selection and evolution of functional proteins by using ribosome display
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Cited by
Directed Evolution: Methodologies and Applications
Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00260 · Cited by 719
Six Classes of Nuclear Localization Signals Specific to Different Binding Grooves of Importin α
Kosugi, S., Hasebe, M., Matsumura, N., Takashima, H., Miyamoto-Sato, E., Tomita, M., & Yanagawa, H. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m807017200 · Cited by 615
Selective Covalent Protein Immobilization: Strategies and Applications
Wong, L. S., Khan, F., & Micklefield, J. (2009) · Chemical Reviews · doi:10.1021/cr8004668 · Cited by 489
Semisynthetic DNA–Protein Conjugates for Biosensing and Nanofabrication
Niemeyer, C. M. (2010) · Angewandte Chemie International Edition · doi:10.1002/anie.200904930 · Cited by 369
In-vitro protein evolution by ribosome display and mRNA display
Lipovsek, D., & Plückthun, A. (2004) · Journal of Immunological Methods · doi:10.1016/j.jim.2004.04.008 · Cited by 353
Metadata
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1997-09-08
- Volume
- 414
- Issue
- 2
- Pages
- 405-408
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2055760720
- Cited by
- 462
- References
- 16
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- bronze
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- In vitro virus: Bonding of mRNA bearing puromycin at the 3′‐terminal end to the C‐terminal end of its encoded protein on the ribosome in vitro. (1997). FEBS Letters. https://doi.org/10.1016/s0014-5793(97)01026-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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