The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Mitochondrial DNA sequences from a 7000-year old brain
Open accessAbstract
Pieces of mitochondrial DNA from a 7000-year-old human brain were amplified by the polymerase chain reaction and sequenced. Albumin and high concentrations of polymerase were required to overcome a factor in the brain extract that inhibits amplification. For this and other sources of ancient DNA, we find an extreme inverse dependence of the amplification efficiency on the length of the sequence to be amplified. This property of ancient DNA distinguishes it from modern DNA and thus provides a new criterion of authenticity for use in research on ancient DNA. The brain is from an individual recently excavated from Little Salt Spring in southwestern Florida and the anthropologically informative sequences it yielded are the first obtained from archaeologically retrieved remains. The sequences show that this ancient individual belonged to a mitochondrial lineage that is rare in the Old World and not previously known to exist among Native Americans. Our finding brings to three the number of maternal lineages known to have been involved in the prehistoric colonization of the New World.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
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Self-citation
Cited by
Identification of the remains of the Romanov family by DNA analysis
Gill, P., Ivanov, P. L., Kimpton, C., Piercy, R., Benson, N., Tully, G., Evett, I., Hagelberg, E., & Sullivan, K. (1994) · Nature Genetics · doi:10.1038/ng0294-130 · Cited by 693
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, T. C. (2012) · Journal of Visualized Experiments · doi:10.3791/3998 · Cited by 493
Orlando, L., Allaby, R., Skoglund, P., Der Sarkissian, C., Stockhammer, P. W., Ávila-Arcos, M. C., Fu, Q., Krause, J., Willerslev, E., Stone, A. C., et al. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-020-00011-0 · Cited by 411
mtDNA Analysis of a Prehistoric Oneota Population: Implications for the Peopling of the New World
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Ancient DNA from a pre‐Columbian Amerindian population
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Human evolution: a tale from ancient genomes
Llamas, B., Willerslev, E., & Orlando, L. (2017) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2015.0484 · Cited by 100
The Domestication Makeup: Evolution, Survival, and Challenges
Ahmad, H. I., Ahmad, M. J., Jabbir, F., Ahmar, S., Ahmad, N., Elokil, A. A., & Chen, J. (2020) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2020.00103 · Cited by 98
Optimization of DNA Recovery and Amplification from Non-Carbonized Archaeobotanical Remains
Wales, N., Andersen, K., Cappellini, E., Ávila-Arcos, M. C., & Gilbert, M. T. P. (2014) · PLoS ONE · doi:10.1371/journal.pone.0086827 · Cited by 80
Nucleic Acid Databases and Molecular-Scale Computing
Song, X., & Reif, J. (2019) · ACS Nano · doi:10.1021/acsnano.9b02562 · Cited by 73
Further evaluation of the efficacy of contamination removal from bone surfaces
Barta, J. L., Monroe, C., & Kemp, B. M. (2013) · Forensic Science International · doi:10.1016/j.forsciint.2013.06.004 · Cited by 33
Metadata
- Authors
- Pääbo, S., Gifford, J. A., & Wilson, A. C.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1988
- Volume
- 16
- Issue
- 20
- Pages
- 9775-9787
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2168536110
- Cited by
- 489
- References
- 3
- FWCI
- 14.31
- Type
- journal article
- Language
- en
- Open access
- bronze
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