The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells
Open accessAbstract
Hypervariable minisatellites can be amplified from human DNA by the polymerase chain reaction, using primers from DNA flanking the minisatellite to amplify the entire block of tandem repeat units. Minisatellite alleles up to 5-10 kb long can be faithfully amplified. At least six minisatellite loci can be co-amplified from the same DNA sample and simultaneously detected to provide a reproducible and highly variable DNA fingerprint which can be obtained from nanogram quantities of human DNA. The polymerase chain reaction can also be used to analyse single target minisatellite molecules and single human cells, despite the appearance of spurious PCR products from some hypervariable loci. DNA fingerprinting at the level of one or a few cells therefore appears possible.
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Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
Mitochondrial DNA sequences in single hairs from a southern African population.
Vigilant, L., Pennington, R., Harpending, H., Kocher, T. D., & Wilson, A. C. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.23.9350 · Cited by 567
DNA polymerase fidelity and the polymerase chain reaction.
Eckert, K. A., & Kunkel, T. A. (1991) · Genome Research · doi:10.1101/gr.1.1.17 · Cited by 448
Zhu, H., Zhang, H., Xu, Y., Laššáková, S., Korabečná, M., & Neužil, P. (2020) · BioTechniques · doi:10.2144/btn-2020-0057 · Cited by 380
Metadata
- Authors
- Jeffreys, A. J., Wilson, V., Neumann, R., & Keyte, J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1988
- Volume
- 16
- Issue
- 23
- Pages
- 10953-10971
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2010933912
- Cited by
- 496
- References
- 2
- FWCI
- 17.79
- Type
- journal article
- Language
- en
- Open access
- green
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- Jeffreys, A. J., Wilson, V., Neumann, R., & Keyte, J. (1988). Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells. Nucleic Acids Research. https://doi.org/10.1093/nar/16.23.10953
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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