The DOI matches its Crossref record.
Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction
Open accessAbstract
A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Three-dimensional structure of a hammerhead ribozyme
Pley, H. W., Flaherty, K. M., & McKay, D. B. (1994) · Nature · doi:10.1038/372068a0 · Cited by 953
Role of divalent metal ions in the hammerhead RNA cleavage reaction
Dahm, S. C., & Uhlenbeck, O. C. (1991) · Biochemistry · doi:10.1021/bi00103a011 · Cited by 366
Self-citationEvidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanism
Dahm, S. C., Derrick, W. B., & Uhlenbeck, O. C. (1993) · Biochemistry · doi:10.1021/bi00211a013 · Cited by 270
Self-citationMECHANISTIC ASPECTS OF ENZYMATIC CATALYSIS: Lessons from Comparison of RNA and Protein Enzymes
Narlikar, G. J., & Herschlag, D. (1997) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.66.1.19 · Cited by 262
RNA structure and NMR spectroscopy
Varani, G., & Tinoco, I. (1991) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583500003875 · Cited by 257
Metadata
- Authors
- Ruffner, D. E., & Uhlenbeck, O. C.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- Volume
- 18
- Issue
- 20
- Pages
- 6025-6029
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1980254677
- Cited by
- 161
- References
- 0
- FWCI
- 4.46
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction. https://app.cochat.ai/papers/10.1093/nar/18.20.6025
- The paper
- Ruffner, D. E., & Uhlenbeck, O. C. (1990). Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction. Nucleic Acids Research. https://doi.org/10.1093/nar/18.20.6025
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
