The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Single base pair mutation analysis by PNA directed PCR clamping
Open accessAbstract
A novel method that allows direct analysis of single base mutation by the polymerase chain reaction (PCR) is described. The method utilizes the finding that PNAs (peptide nucleic acids) recognize and bind to their complementary nucleic acid sequences with higher thermal stability and specificity than the corresponding deoxyribooligonucleotides and that they cannot function as primers for DNA polymerases. We show that a PNA/DNA complex can effectively block the formation of a PCR product when the PNA is targeted against one of the PCR primer sites. Furthermore, we demonstrate that this blockage allows selective amplification/suppression of target sequences that differ by only one base pair. Finally we show that PNAs can be designed in such a way that blockage can be accomplished when the PNA target sequence is located between the PCR primers.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide
Nielsen, P. E., Egholm, M., Berg, R. H., & Buchardt, O. (1991) · Science · doi:10.1126/science.1962210 · Cited by 3,332
Self-citation
Cited by
Peptide nucleic acid (PNA): its medical and biotechnical applications and promise for the future
Ray, A., & Nordén, B. (2000) · The FASEB Journal · doi:10.1096/fasebj.14.9.1041 · Cited by 416
Solid‐Phase synthesis of peptide nucleic acids
Christensen, L., Fitzpatrick, R., Gildea, B., Petersen, K. H., Hansen, H. F., Koch, T., Egholm, M., Buchardt, O., Nielsen, P. E., Coull, J., et al. (1995) · Journal of Peptide Science · doi:10.1002/psc.310010304 · Cited by 337
Self-citationSong, P., Wu, L. R., Yan, Y. H., Zhang, J. X., Chu, T., Kwong, L. N., Patel, A. A., & Zhang, D. Y. (2022) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00837-3 · Cited by 319
An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum
Das, J., Ivanov, I., Montermini, L., Rak, J., Sargent, E. H., & Kelley, S. O. (2015) · Nature Chemistry · doi:10.1038/nchem.2270 · Cited by 274
Triple helix formation and the antigene strategy for sequence-specific control of gene expression
Praseuth, D., Guieysse, A. L., & Hélène, C. (1999) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(99)00149-9 · Cited by 262
Metadata
- Authors
- Ørum, H., Nielsen, P. E., Egholm, M., Berg, R. H., Buchardt, O., & Stanley, C.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1993
- Volume
- 21
- Issue
- 23
- Pages
- 5332-5336
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2055245741
- Cited by
- 334
- References
- 2
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Single base pair mutation analysis by PNA directed PCR clamping. https://app.cochat.ai/papers/10.1093/nar/21.23.5332
- The paper
- Ørum, H., Nielsen, P. E., Egholm, M., Berg, R. H., Buchardt, O., & Stanley, C. (1993). Single base pair mutation analysis by PNA directed PCR clamping. Nucleic Acids Research. https://doi.org/10.1093/nar/21.23.5332
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
