Cited by 238RNA and protein synthesis mechanismsRNA Research and SplicingAnimal Virus Infections Studies

Comparison of Picornaviral IRES-Driven Internal Initiation of Translation in Cultured Cells of Different Origins

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Borman, A. M., Le Mercier, P., Girard, M., Kean, K. M.·1997·Nucleic Acids Research

Abstract

We recently compared the efficiency of six picornaviral internal ribosome entry segments (IRESes) and the hepatitis C virus (HCV) IRES for their ability to drive internal initiation of translationin vitro. Here we present the results of a similar comparison performed in six different cultured cell lines infected with a recombinant vaccinia virus expressing the T7 polymerase and transfected with dicistronic plasmids. The IRESes could be divided into three groups: (i) the cardiovirus and aphthovirus IRESes (and the HCV element) direct internal initiation efficiently in all cell lines tested; (ii) the enterovirus and rhinovirus IRESes are at least equally efficient in several cell lines, but are extremely inefficient in certain cell types; and (iii) the hepatitis A virus IRES is incapable of directing efficient internal initiation in any of the cell lines used (including human hepatocytes). These are the same three groups found when IRESes were classified according to their activitiesin vitro, or according to sequence homologies. In a mouse neuronal cell line, the poliovirus and other type I IRESes were not functional in an artificial bicistronic context. However, infectious poliovirions were produced efficiently after transfection of these cells with a genomic length RNA. Furthermore, activity of the type I IRESes was dramatically increased upon co-expression of the poliovirus 2A proteinase, demonstrating that while IRES efficiency may vary considerably from one cell type to another, at least in some cases viral proteins are capable of overcoming cell-specific translational defects.

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Crossref · 10.1093/nar/25.5.925

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Authors
Borman, A. M., Le Mercier, P., Girard, M., & Kean, K. M.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Kean, K. M.
Venue
Nucleic Acids Research
Published
1997-03-01
Volume
25
Issue
5
Pages
925-932
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2161943011
Cited by
238
References
49
Citations by year
2026: 4 · 2025: 4 · 2024: 7 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 4 · 2019: 5 · 2018: 6 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 8 · 2013: 5 · 2012: 5
FWCI
6.40
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
Keywords
internal ribosome entry site, internal translation initiation, poliovirus, hepatitis C virus, vaccinia virus
MeSH terms
Amino Acid Sequence, Animals, Haplorhini, Base Sequence, Cell Line, Cysteine Endopeptidases, Cricetinae, HeLa Cells, Humans, Molecular Sequence Data, Peptide Chain Initiation, Translational, Picornaviridae, RNA, Viral, Transfection, Tumor Cells, Cultured, Viral Proteins, Xenopus laevis, Gene Expression Regulation, Viral, Mice

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