Interaction of tetracycline with RNA: photoincorporation into ribosomal RNA of Escherichia coli
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Photolysis of [3H]tetracycline in the presence of Escherichia coli ribosomes results in an approximately 1:1 ratio of labelling ribosomal proteins and RNAs. In this work we characterize crosslinks to both 16S and 23S RNAs. Previously, the main target of photoincorporation of [3H]tetracycline into ribosomal proteins was shown to be S7, which is also part of the one strong binding site of tetracycline on the 30S subunit. The crosslinks on 23S RNA map exclusively to the central loop of domain V (G2505, G2576 and G2608) which is part of the peptidyl transferase region. However, experiments performed with chimeric ribosomal subunits demonstrate that peptidyltransferase activity is not affected by tetracycline crosslinked solely to the 50S subunits. Three different positions are labelled on the 16S RNA, G693, G1300 and G1338. The positions of these crosslinked nucleotides correlate well with footprints on the 16S RNA produced either by tRNA or the protein S7. This suggests that the nucleotides are labelled by tetracycline bound to the strong binding site on the 30S subunit. In addition, our results demonstrate that the well known inhibition of tRNA binding to the A-site is solely due to tetracycline crosslinked to 30S subunits and furthermore suggest that interactions of the antibiotic with 16S RNA might be involved in its mode of action.
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Biochemical studies have implicated both ribosomal proteins and 16S RNA in tetracycline binding.
“Biochemical studies have implicated both ribosomal proteins and 16S RNA in tetracycline binding (Moazed and Noller 1987; Buck and Cooperman 1990; Oehler et al. 1997”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Oehler et al. 1997
A direct cross-link from tetracycline to G890 has been observed at high tetracycline concentrations.
“A direct cross-link from Tc to G890 has been observed at high Tc concentrations (Oehler et al. 1997”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Oehler et al. 1997
Cites
Goldman, R. A., Hasan, T., Hall, C. C., Strycharz, W. A., & Cooperman, B. S. (1983) · Biochemistry · doi:10.1021/bi00271a020 · Cited by 134 · see citing works
Epe, B., Woolley, P., & Hornig, H. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)81539-9 · Cited by 50 · see citing works
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Chopra, I., & Roberts, M. (2001) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.65.2.232-260.2001 · Cited by 4,517 · see citing works
Brodersen, D. E., Clemons, W. M., Carter, A. P., Morgan-Warren, R. J., Wimberly, B. T., & Ramakrishnan, V. (2000) · Cell · doi:10.1016/s0092-8674(00)00216-6 · Cited by 950 · see citing works
“The inhibition of translocation is accompanied and Noller, 1987; Buck and Cooperman, 1990; Oehler et al., 1997).”
BackgroundCrystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3
Pioletti, M., Schlünzen, F., Harms, J., Zarivach, R., Glühmann, M., Avila, H., Bashan, A., Bartels, H., Auerbach, T., Jacobi, C., et al. (2001) · The EMBO Journal · doi:10.1093/emboj/20.8.1829 · Cited by 527 · see citing works
Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance
Connell, S. R., Tracz, D. M., Nierhaus, K. H., & Taylor, D. E. (2003) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.47.12.3675-3681.2003 · Cited by 449 · see citing works
16S rRNA Mutation Associated with Tetracycline Resistance in a Gram-Positive Bacterium
Ross, J. I., Eady, E. A., Cove, J. H., & Cunliffe, W. J. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.7.1702 · Cited by 194 · see citing works
Mutations in the 16S rRNA Genes of<i>Helicobacter pylori</i>Mediate Resistance to Tetracycline
Trieber, C. A., & Taylor, D. E. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.8.2131-2140.2002 · Cited by 174 · see citing works
Target- and resistance-based mechanistic studies with TP-434, a novel fluorocycline antibiotic
Grossman, T. H., Starosta, A. L., Fyfe, C., O'Brien, W., Rothstein, D. M., Mikolajka, A., Wilson, D. N., & Sutcliffe, J. A. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.06187-11 · Cited by 162 · see citing works
Spahn, C. M. T., Blaha, G., Agrawal, R. K., Penczek, P., Grassucci, R. A., Trieber, C. A., Connell, S. R., Taylor, D. E., Nierhaus, K. H., & Frank, J. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00238-6 · Cited by 81 · see citing works
Noah, J. W., Dolan, M. A., Babin, P., & Wollenzien, P. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.23.16576 · Cited by 46 · see citing works
- Authors
- Oehler, R.
- Affiliations
- University of Vienna (AT)
- Corresponding author
- Oehler, R
- ORCID
- Oehler, R
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-03-15
- Volume
- 25
- Issue
- 6
- Pages
- 1219-1224
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC146554
- OpenAlex
- W1968311976
- Cited by
- 66
- References
- 2
- Citations by year
- 2026: 1 · 2025: 5 · 2023: 3 · 2022: 2 · 2021: 1 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 5 · 2015: 1 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- tetracycline, ribosomal RNA, 16S rRNA, 23S rRNA, Escherichia coli ribosome, 30S subunit, antibiotic binding sites, antibiotic mechanism of action, crosslinking
- MeSH terms
- Base Sequence, Binding Sites, Chimera, Cross-Linking Reagents, Escherichia coli, Models, Structural, Molecular Sequence Data, Nucleic Acid Conformation, Photolysis, RNA-Directed DNA Polymerase, Ribosomes, RNA, Bacterial, RNA, Ribosomal, RNA, Ribosomal, 16S, RNA, Ribosomal, 23S, Tetracycline
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