Competition between tetracycline and tRNA at both P and A sites of the ribosome of Escherichia coli
Abstract
Fluorescence anisotropy studies performed on 6‐demethylchlortetracycline, binding to the ribosome of E. coli in competition with tRNA at the P site or at both P and A sites, have provided a quantitative assessment in situ of the interaction of this antibiotic with the A site and have demonstrated that there is also an interaction between tetracycline and the P site.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Tetracycline has one primary and multiple secondary binding sites within the small subunit.
“Tc has one primary and multiple secondary binding sites within the small subunit (Epe et al. 1987; Kolesnikov et al. 1996”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Epe et al. 1987
Tetracycline binding prevents an entering aminoacyl-tRNA from being recognized by the codon in the mRNA and thereby incapacitates the ribosome for protein synthesis.
“Thus, Tc binding prevents any entering aminoacyl-tRNA from being recognized by the codon in the messenger RNA and thereby incapacitates the ribosome for protein synthesis 3.”
From Mechanism of tetracycline resistance by ribosomal protection protein Tet(O) (2013) · cites this paper as [3]
Cites
Structure, Function, and Genetics of Ribosomes
Hardesty, B. D., & Kramer, G. (1986) · Springer series in molecular biology · doi:10.1007/978-1-4612-4884-2 · Cited by 852 · see citing works
[41] Reconstitution of ribosomes from subribosomal components
Traub, P., Mizushima, S.-I., Lowry, C. V., & Nomura, M. (1971) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(71)20043-4 · Cited by 290 · see citing works
Bacterial protein synthesis: The effects of antibiotics
Cundliffe, E., & McQuillen, K. (1967) · Journal of Molecular Biology · doi:10.1016/0022-2836(67)90249-5 · Cited by 168 · see citing works
Epe, B., & Woolley, P. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb01771.x · Cited by 44 · see citing works
Self-citationStructure of Ribosomal Protein L6 from Escherichia coli
Steinhäuser, K. G., Woolley, P., Epe, B., & Dijk, J. (1982) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1982.tb06913.x · Cited by 16 · see citing works
Self-citationHornig, H., Woolley, P., & Lührmann, R. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91061-6 · Cited by 9 · see citing works
Self-citationLührmann, R. (1980) · Nucleic Acids Research · doi:10.1093/nar/8.23.5813 · Cited by 8 · see citing works
Hornig, H., Woolley, P., & Lührmann, R. (1983) · FEBS Letters · doi:10.1016/0014-5793(83)80519-5 · Cited by 6 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Brodersen, D. E., Clemons, W. M., Carter, A. P., Morgan-Warren, R. J., Wimberly, B. T., & Ramakrishnan, V. (2000) · Cell · doi:10.1016/s0092-8674(00)00216-6 · Cited by 950 · see citing works
“…secondary by an increase in the affinity of the A site for aminoacyl-tRNA (Eustice and Wilhelm, 1984a). binding sites within the small subunit (Epe et al., 1987; Kolesnikov et al., 1996), but the relevance of the second-Here we report the three-dimensional structures of the 30S ribosomal…”
BackgroundBacterial resistance to tetracycline: mechanisms, transfer, and clinical significance
Speer, B. S., Shoemaker, N. B., & Salyers, A. A. (1992) · Clinical Microbiology Reviews · doi:10.1128/cmr.5.4.387 · Cited by 511 · see citing works
Tetracyclines: Antibiotic action, uptake, and resistance mechanisms
Schnappinger, D., & Hillen, W. (1996) · Archives of Microbiology · doi:10.1007/s002030050339 · Cited by 484 · see citing works
Resistance mechanisms in Campylobacter jejuni
Iovine, N. M. (2013) · Virulence · doi:10.4161/viru.23753 · Cited by 274 · see citing works
A tetracycline-binding RNA aptamer
Berens, C., Thain, A., & Schroeder, R. (2001) · Bioorganic & Medicinal Chemistry · doi:10.1016/s0968-0896(01)00063-3 · Cited by 199 · see citing works
Electrochemical aptasensor for tetracycline detection
Kim, Y.-J., Kim, Y. S., Niazi, J. H., & Gu, M. B. (2009) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-009-0371-4 · Cited by 191 · see citing works
Mechanism of tetracycline resistance by ribosomal protection protein Tet(O)
Li, W., Atkinson, G. C., Thakor, N. S., Allas, Ü., Lu, C.-C., Chan, K.-Y., Tenson, T., Schulten, K., Wilson, K. S., Hauryliuk, V., et al. (2013) · Nature Communications · doi:10.1038/ncomms2470 · Cited by 147 · see citing works
Antimicrobial Resistance in Campylobacter spp
Shen, Z., Wang, Y., Zhang, Q., & Shen, J. (2018) · Microbiology Spectrum · doi:10.1128/microbiolspec.arba-0013-2017 · Cited by 126 · see citing works
Wang, S., Yong, W., Liu, J., Zhang, L., Chen, Q., & Dong, Y. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.02.032 · Cited by 121 · see citing works
Guillaume, G., Verbrugge, D., Chasseur-Libotte, M. L., Moens, W., & Collard, J. (2000) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2000.tb00701.x · Cited by 120 · see citing works
Antibiotic drugs targeting bacterial RNAs
Hong, W., Zeng, J., & Xie, J. (2014) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2014.06.012 · Cited by 107 · see citing works
Monitoring and detection of antibiotic residues in animal derived foods: Solutions using aptamers
Liu, Y., Deng, Y., Li, S., Chow, F. W., Liu, M., & He, N. (2022) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2022.04.008 · Cited by 92 · see citing works
Trieber, C. A., Burkhardt, N., Nierhaus, K. H., & Taylor, D. E. (1998) · bchm · doi:10.1515/bchm.1998.379.7.847 · Cited by 68 · see citing works
Interaction of tetracycline with RNA: photoincorporation into ribosomal RNA of Escherichia coli
Oehler, R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.6.1219 · Cited by 66 · see citing works
Buck, M. A., & Cooperman, B. S. (1990) · Biochemistry · doi:10.1021/bi00474a024 · Cited by 57 · see citing works
Sarecycline interferes with tRNA accommodation and tethers mRNA to the 70S ribosome
Batool, Z., Lomakin, I. B., Polikanov, Y. S., & Bunick, C. G. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2008671117 · Cited by 50 · see citing works
Ligand-induced conformational capture of a synthetic tetracycline riboswitch revealed by pulse EPR
Wunnicke, D., Strohbach, D., Weigand, J. E., Appel, B., Feresin, E., Suess, B., Müller, S., & Steinhoff, H. (2010) · RNA · doi:10.1261/rna.2222811 · Cited by 51 · see citing works
Bortolotto, T., Silva, P. P., Neves, A., Pereira-Maia, E. C., & Terenzi, H. F. (2011) · Inorganic Chemistry · doi:10.1021/ic201349s · Cited by 51 · see citing works
Noah, J. W., Dolan, M. A., Babin, P., & Wollenzien, P. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.23.16576 · Cited by 46 · see citing works
Interactions of tetracycline and its derivatives with DNA in vitro in presence of metal ions
Khan, M. A., & Musarrat, J. (2003) · International Journal of Biological Macromolecules · doi:10.1016/s0141-8130(03)00066-7 · Cited by 42 · see citing works
Decoding at the ribosomal A site: antibiotics, misreading and energy of aminoacyl-tRNA binding
Hornig, H., Woolley, P., & Lührmann, R. (1987) · Biochimie · doi:10.1016/0300-9084(87)90207-0 · Cited by 40 · see citing works
Self-citationBinding Interaction between Tet(M) and the Ribosome: Requirements for Binding
Dantley, K. A., Dannelly, H. K., & Burdett, V. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.16.4089-4092.1998 · Cited by 37 · see citing works
tRNA modification activity is necessary for Tet(M)-mediated tetracycline resistance
Burdett, V. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.22.7209-7215.1993 · Cited by 36 · see citing works
Mukherjee, R., Patra, M., Dutta, D., Banik, M., & Basu, T. (2016) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2016.06.006 · Cited by 35 · see citing works
Dou, X., Wu, Q., Luo, S., Yang, J., Dong, B., Wang, L., Qu, H., & Zheng, L. (2024) · Talanta · doi:10.1016/j.talanta.2024.125702 · Cited by 34 · see citing works
The Molecular Mechanisms of Tetracycline Resistance in the Pneumococcus
Widdowson, C. A., & Klugman, K. P. (1998) · Microbial Drug Resistance · doi:10.1089/mdr.1998.4.79 · Cited by 32 · see citing works
Li, S., Liu, J., Sun, K., Yang, Z., & Ling, W. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.114063 · Cited by 20 · see citing works
Drysdale, M. J., Lentzen, G., Matassova, N., Murchie, A. I. H., Aboul‐ela, F., & Afshar, M. (2002) · Progress in medicinal chemistry · doi:10.1016/s0079-6468(08)70069-9 · Cited by 20 · see citing works
The role of adjuvants in overcoming antibacterial resistance due to enzymatic drug modification
El-Khoury, C., Mansour, E., Yuliandra, Y., Lai, F., Hawkins, B. A., Du, J. J., Sundberg, E. J., Sluis‐Cremer, N., Hibbs, D. E., & Groundwater, P. W. (2022) · RSC Medicinal Chemistry · doi:10.1039/d2md00263a · Cited by 19 · see citing works
Atomic Structures of the 30S Subunit and Its Complexes with Ligands and AntibioticsPreprint
Brodersen, D. E., Carter, A. P., Clemons, W., Morgan-Warren, R. J., Murphy, F. V., Ogle, J. M., Tarry, M. J., Wimberly, B. T., & Ramakrishnan, V. (2001) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2001.66.17 · Cited by 19 · see citing works
Inhibition of nuclear pre‐mRNA splicing by antibiotics in vitro
Hertweck, M., Hiller, R., & Mueller, M. W. (2002) · European Journal of Biochemistry · doi:10.1046/j.0014-2956.2001.02636.x · Cited by 16 · see citing works
Mukherjee, R., Dutta, D., Patra, M., Chatterjee, B., & Basu, T. (2018) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2018.11.004 · Cited by 15 · see citing works
Skalnik, C. J., Cheah, S., Yang, M. Y., Wolff, M., Spangler, R. K., Talman, L., Morrison, J. H., Peirce, S. M., Agmon, E., & Covert, M. W. (2023) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1011232 · Cited by 14 · see citing works
Construction of ratiometric fluorescent sensors by ribonucleopeptides
Annoni, C., Nakata, E., Tamura, T., Liew, F. F., Nakano, S., Gelmi, M. L., & Morii, T. (2012) · Organic & Biomolecular Chemistry · doi:10.1039/c2ob26722e · Cited by 12 · see citing works
Antimicrobial Resistance and Campylobacter jejuni and C. coli
Rossi, M., Olkkola, S., Roasto, M., Kivistö, R. I., & Hänninen, M. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-801214-7.00004-1 · Cited by 8 · see citing works
Target Modification As A Mechanism Of Antimicrobial Resistance
Hooper, D. C. (2001) · CRC Press eBooks · doi:10.1201/9780824744939.ch7 · Cited by 8 · see citing works
Liu, J., Phelps, G. A., DUNN, C., Murphy, P. A., Wilt, L. A., Loudon, V. C., Lee, R. B., Fernando, D. M., Yang, L., Tran, K. N., et al. (2024) · Tuberculosis · doi:10.1016/j.tube.2024.102592 · Cited by 5 · see citing works
Tetracyclines induce changes in accessibility of ribosomal proteins to proteases
Kolesnikov, I. (1996) · Biochimie · doi:10.1016/s0300-9084(97)84340-4 · Cited by 5 · see citing works
Fluorimetric distance determination by resonance energy transfer
FRIEDRICH, K., Woolley, P., & Steinh�user, K. G. (1988) · European Biophysics Journal · doi:10.1007/bf00255325 · Cited by 4 · see citing works
Self-citationAntimicrobial Resistance inCampylobacterspp.
Shen, Z., Wang, Y., Zhang, Q., & Shen, J. (2018) · ASM Press eBooks · doi:10.1128/9781555819804.ch14 · Cited by 4 · see citing works
Sheng, T., Chen, H., Lei, Y., Zhang, B., & Zhu, H. (2023) · Analytical Methods · doi:10.1039/d3ay01072d · Cited by 3 · see citing works
Zhang, L., Jin, T., Sathiyaseelan, A., Lu, Y., Zhang, X., & Wang, M. (2025) · Inorganic Chemistry Communications · doi:10.1016/j.inoche.2025.114428 · Cited by 3 · see citing works
Tetracycline Antibiotics and Novel Analogs
Genilloud, O., & Vicente, F. (2013) · Springer eBooks · doi:10.1007/978-3-642-39968-8_12 · Cited by 3 · see citing works
Strahilevitz, J., & Hooper, D. C. (2009) · Wiley eBooks · doi:10.1002/9781444308464.ch10 · Cited by 1 · see citing works
Peptidyltransfer Reaction Catalyzed by the Ribosome and the Ribozyme: a Dissertation
Sun, L. (2003) · The Journal of the American Medical Association (JAMA) Network (American Medical Association) · doi:10.13028/6ds0-4c54
Drug-induced bone regeneration in a diabetic model
da Silva, J. C. O. R. (2017) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) · doi:10.34626/2kh7-wb32
Sutcliffe, J. A., & Roberts, M. C. (2014) · ASM Press eBooks · doi:10.1128/9781555817572.ch23
Target Modification as a Mechanism of Antimicrobial Resistance
Hooper, D. C. (2007) · CRC Press eBooks · doi:10.1201/9781420008753.ch7
Lee, R. E., Jr., Liu, J., Phelps, G. A., DUNN, C., Murphy, P. A., Wilt, L. A., Loudon, V. C., Lee, R. B., Fernando, D., Yang, L., et al. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4965130
- Authors
- Epe, B., Woolley, P., & Hornig, H.
- Affiliations
- University of Würzburg (DE)
- Aarhus University (DK)
- Max Planck Institute for Molecular Genetics (DE)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1987-03-23
- Volume
- 213
- Issue
- 2
- Pages
- 443-447
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2075781111
- Cited by
- 50
- References
- 8
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 2 · 2022: 2 · 2020: 2 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 3 · 2012: 1
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- tetracycline, tRNA, ribosome, P site, A-site, antibiotic binding, Escherichia coli, fluorescence anisotropy
- MeSH terms
- Binding Sites, Binding, Competitive, Demeclocycline, Escherichia coli, Fluorescence Polarization, Ribosomes, RNA, Transfer, RNA, Transfer, Amino Acyl, RNA, Transfer, Met
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
