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Thermodynamics of internal C.T mismatches in DNA

Open access
Allawi, H.·1998·Nucleic Acids Research

Abstract

Thermodynamics of 23 oligonucleotides with internal single C.T mismatches were obtained by measuring UV absorbance as a function of temperature. Results from these 23 duplexes were combined with three measurements from the literature to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal C.T mismatches. The data show that the nearest-neighbor model is adequate for predicting thermodynamics of oligonucleotides with internal C.T with average deviations for Delta G degrees37, Delta H degrees, Delta S degrees and T m of 6.4%, 9.9%, 10.6%, and 1.9 degreesC respectively. C.T mismatches destabilize the duplex in all sequence contexts. The thermodynamic contribution of C. T mismatches to duplex stability varies weakly depending on the orientation of the mismatch and its context and ranges from +1.02 kcal/mol for GCG/CTC and CCG/GTC to +1.95 kcal/mol for TCC/ATG.

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Metadata

Authors
Allawi, H.
Venue
Nucleic Acids Research
Published
1998-06-01
Volume
26
Issue
11
Pages
2694-2701
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2116791963
Cited by
234
References
4
FWCI
2.36
Type
journal article
Language
en
Open access
green

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Allawi, H. (1998). Thermodynamics of internal C.T mismatches in DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/26.11.2694

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