Cited by 104DNA Repair MechanismsLight effects on plantsPlant Molecular Biology Research

DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells

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Kimura, S.·2004·Nucleic Acids Research

Abstract

We investigated expression patterns of DNA repair genes such as the CPD photolyase, UV-DDB1, CSB, PCNA, RPA32 and FEN-1 genes by northern hybridization analysis and in situ hybridization using a higher plant, rice (Oryza sativa L. cv. Nipponbare). We found that all the genes tested were expressed in tissues rich in proliferating cells, but only CPD photolyase was expressed in non-proliferating tissue such as the mature leaves and elongation zone of root. The removal of DNA damage, cyclobutane pyrimidine dimers and (6-4) photoproducts, in both mature leaves and the root apical meristem (RAM) was observed after UV irradiation under light. In the dark, DNA damage in mature leaves was not repaired efficiently, but that in the RAM was removed rapidly. Using a rice 22K custom oligo DNA microarray, we compared global gene expression patterns in the shoot apical meristem (SAM) and mature leaves. Most of the excision repair genes were more strongly expressed in SAM. These results suggested that photoreactivation is the major DNA repair pathway for the major UV-induced damage in non-proliferating cells, while both photoreactivation and excision repair are active in proliferating cells.

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Crossref · 10.1093/nar/gkh591

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  • BER and NER, but not photoreactivation, are active in proliferating cells of higher plants.

    “Recently, it has been reported that the mechanisms of BER and NER (but not photoreactivation) in higher plants are active in proliferating cells [172].”

    From Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair (2010) · cites this paper as [172]

    The paper supports excision repair in proliferating cells, but explicitly contradicts the exclusion of photoreactivation. The Abstract (p. 1) says both photoreactivation and excision repair are active in proliferating cells; the Discussion (p. 5) likewise says the meristem may need both. (LLM, conf=0.91)

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  • Photoreactivation mediated by photolyases is thought to be the major DNA repair pathway in several higher plants such as rice, Arabidopsis, wheat, and maize.

    “Photoreactivation mediated by the enzyme photolyases is thought to be the major DNA repair pathway in several higher plants such as rice, Arabidopsis, wheat, and maize [170–172].”

    From Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair (2010) · cites this paper as [172]

    The Introduction (p. 1) says photolyase-mediated photoreactivation is thought to be the major repair pathway for two types of UV-induced DNA lesion in higher plants. It does not establish that claim separately for rice, Arabidopsis, wheat, and maize, and the Discussion (p. 5) limits the paper’s own finding to non-proliferating rice leaves rather than DNA repair generally. (LLM, conf=0.84)

    Experimental — accuracy not yet measured
  • Genes required for dark repair have been identified in several plant species.

    “In several plant species, some of the genes requisite for dark repair have been identified [171, 172, 202–204].”

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Authors
Kimura, S.
Affiliations
Tokyo University of Science (JP)
Corresponding author
Kimura, S.
Venue
Nucleic Acids Research
Published
2004-05-17
Volume
32
Issue
9
Pages
2760-2767
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2170811853
Cited by
103
References
48
Citations by year
2026: 2 · 2025: 1 · 2024: 5 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 6 · 2017: 3 · 2016: 5 · 2015: 3 · 2014: 5 · 2013: 2 · 2012: 3
FWCI
2.49
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
DNA repair, photoreactivation, excision repair, nucleotide excision repair, base excision repair, CPD photolyase, CSB, PCNA, RPA32, FEN1, rice, UV-induced DNA damage, cyclobutane pyrimidine dimers, 6-4 photoproducts, root apical meristem, shoot apical meristem, gene expression patterns, proliferating cells, non-proliferating cells
MeSH terms
Cell Division, DNA Damage, DNA Repair, Molecular Sequence Data, Pyrimidine Dimers, Oryza, RNA, Messenger, Genes, Plant, Plant Structures, Meristem, RNA, Plant, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling

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