Cited by 932DNA Repair MechanismsCarcinogens and Genotoxicity AssessmentSkin Protection and Aging

Ultraviolet radiation-mediated damage to cellular DNA

Cadet, Jean, Sage, Evelyne, Douki, Thierry·2005·Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Abstract

Emphasis is placed in this review article on recent aspects of the photochemistry of cellular DNA in which both the UVB and UVA components of solar radiation are implicated individually or synergistically. Interestingly, further mechanistic insights into the UV-induced formation of DNA photoproducts were gained from the application of new accurate and sensitive chromatographic and enzymic assays aimed at measuring base damage. Thus, each of the twelve possible dimeric photoproducts that are produced at the four main bipyrimidine sites can now be singled out as dinucleoside monophosphates that are enzymatically released from UV-irradiated DNA. This was achieved using a recently developed high-performance liquid chromatography-tandem mass spectrometry assay (HPLC-MS/MS) assay after DNA extraction and appropriate enzymic digestion. Interestingly, a similar photoproduct distribution pattern is observed in both isolated and cellular DNA upon exposure to low doses of either UVC or UVB radiation. This applies more specifically to the DNA of rodent and human cells, the cis-syn cyclobutadithymine being predominant over the two other main photolesions, namely thymine-cytosine pyrimidine (6-4) pyrimidone adduct and the related cyclobutyl dimer. UVA-irradiation was found to generate cyclobutane dimers at TT and to a lower extent at TC sites as a likely result of energy transfer mechanism involving still unknown photoexcited chromophore(s). Oxidative damage to DNA is also induced although less efficiently by UVA-mediated photosensitization processes that mostly involved 1O2 together with a smaller contribution of hydroxyl radical-mediated reactions through initially generated superoxide radicals.

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Crossref · 10.1016/j.mrfmmm.2004.09.012

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Authors
Cadet, J., Sage, E., & Douki, T.
Affiliations
Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
Venue
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Publisher
Elsevier BV
Published
2005-04
Volume
571
Issue
1-2
Pages
3-17
ISSN
  • 0027-5107 (ISSN-L)
  • 1386-1964
OpenAlex
W2056826119
Cited by
932
References
121
Citations by year
2026: 10 · 2025: 26 · 2024: 27 · 2023: 26 · 2022: 29 · 2021: 37 · 2020: 46 · 2019: 67 · 2018: 53 · 2017: 38 · 2016: 56 · 2015: 46 · 2014: 59 · 2013: 59 · 2012: 56
FWCI
3.27
Type
journal article
Language
en
Open access
closed
Locations
  • Mutation research. Fundamental and molecular mechanisms of mutagenesis (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
Les Laboratories Pierre Fabre
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
Keywords
ultraviolet radiation, DNA photoproducts, UV-B radiation, UVA, cyclobutane dimers, dinucleoside monophosphates, photosensitization, singlet oxygen, hydroxyl radicals, superoxide, oxidative DNA damage, energy transfer
MeSH terms
Animals, DNA, DNA Damage, Humans, Ultraviolet Rays

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