The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Global transcriptional control by glucose and carbon regulator CcpA in Clostridium difficile
Open accessAbstract
The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. By whole transcriptome analyses, we characterized glucose-dependent and CcpA-dependent gene regulation in Clostridium difficile. About 18% of all C. difficile genes are regulated by glucose, for which 50% depend on CcpA for regulation. The CcpA regulon comprises genes involved in sugar uptake, fermentation and amino acids metabolism, confirming the role of CcpA as a link between carbon and nitrogen pathways. Using combination of chromatin immunoprecipitation and genome sequence analysis, we detected 55 CcpA binding sites corresponding to ∼140 genes directly controlled by CcpA. We defined the C. difficile CcpA consensus binding site (cre(CD) motif), that is, 'RRGAAAANGTTTTCWW'. Binding of purified CcpA protein to 19 target cre(CD) sites was demonstrated by electrophoretic mobility shift assay. CcpA also directly represses key factors in early steps of sporulation (Spo0A and SigF). Furthermore, the C. difficile toxin genes (tcdA and tcdB) and their regulators (tcdR and tcdC) are direct CcpA targets. Finally, CcpA controls a complex and extended regulatory network through the modulation of a large set of regulators.
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Cites
Carbon catabolite repression in bacteria: many ways to make the most out of nutrients
Görke, B., & Stülke, J. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1932 · Cited by 1,701
A comparison of background correction methods for two-colour microarrays
Ritchie, M. E., Silver, J., Oshlack, A., Holmes, M., Diyagama, D., Holloway, A., & Smyth, G. K. (2007) · Bioinformatics · doi:10.1093/bioinformatics/btm412 · Cited by 907
Camiade, E., Peltier, J., Bourgeois, I., Couture-Tosi, E., Courtin, P., Antunes, A., Chapot-Chartier, M.-P., Dupuy, B., & Pons, J.-L. (2010) · Journal of Bacteriology · doi:10.1128/jb.01546-09 · Cited by 35
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Cited by
Clostridium difficile infection
Smits, W. K., Lyras, D., Lacy, D. B., Wilcox, M. H., & Kuijper, E. J. (2016) · Nature Reviews Disease Primers · doi:10.1038/nrdp.2016.20 · Cited by 853
Antibiotic perturbations to the gut microbiome
Fishbein, S. R. S., Mahmud, B., & Dantas, G. (2023) · Nature Reviews Microbiology · doi:10.1038/s41579-023-00933-y · Cited by 440
Scaria, J., Suzuki, H., Ptak, C. P., Chen, J.-W., Zhu, Y., Guo, X.-K., & Chang, Y.-F. (2015) · BMC Genomics · doi:10.1186/s12864-015-1663-5 · Cited by 26
Molecular and Functional Analysis of the Type IV Pilus Gene Cluster in Streptococcus sanguinis SK36
Chen, Y.-Y. M., Chiang, Y.-C., Tseng, T.-Y., Wu, H.-Y., Chen, Y.-Y., Wu, C.-H., & Chiu, C.-H. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.02788-18 · Cited by 13
Metadata
- Authors
- Antunes, A., Camiade, E., Monot, M., Courtois, E., Barbut, F., Sernova, N. V., Rodionov, D. A., Martin-Verstraete, I., & Dupuy, B.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-09-18
- Volume
- 40
- Issue
- 21
- Pages
- 10701-10718
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2139520476
- Cited by
- 204
- References
- 3
- FWCI
- 7.26
- Type
- journal article
- Language
- en
- Open access
- gold
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