Cited by 55Genomics and Chromatin DynamicsRNA Research and SplicingCancer-related molecular mechanisms research

Regulation of anti-sense transcription by Mot1p and NC2 via removal of TATA-binding protein (TBP) from the 3′-end of genes

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Koster, Maria J.E., Timmers, H.Th. Marc·2015·Nucleic Acids Research

Abstract

The activity and dynamic nature of TATA-binding protein (TBP) crucial to RNA polymerase II-mediated transcription is under control of the Mot1p and NC2 complexes. Here we show that both TBP regulatory factors play 'hidden' roles in ensuring transcription fidelity by restricting anti-sense non-coding RNA (ncRNA) synthesis. Production of anti-sense ncRNA transcripts is suppressed by Mot1p- and NC2-mediated release of TBP from binding sites at the 3'-end of genes. In this, Mot1p and NC2 collaborate with the Nrd1p-Nab3p-Sen1p (NNS) complex that terminates the synthesis of anti-sense ncRNAs. In several cases anti-sense ncRNA expression interferes with expression of the cognate sense transcript. Our data reveal a novel regulatory mechanism to suppress anti-sense ncRNA expression and pre-initiation complex (PIC) formation at spurious sites.

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  • PCR is an exponential process, so small random variations early on in amplification can have a large impact on distribution.

    “PCR is an exponential process, so small random variations early on in amplification can have a large impact on distribution18–24.”

    From Quantifying Molecular Bias in DNA Data Storage (2019) · cites this paper as [24]

    The Materials and Methods sections on plasmids and chromatin immunoprecipitation describe using PCR to generate fragments and analyze samples. Neither those passages nor searches for exponential amplification and early-cycle stochastic variation address whether small random variations can substantially change the resulting distribution. Page numbers are unavailable in our copy. (LLM, conf=0.76)

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Authors
Koster, M. J. E., & Timmers, H. T. M.
Affiliations
University Medical Center Utrecht (NL)
Corresponding author
Timmers, H.Th. Marc
Venue
Nucleic Acids Research
Published
2014-11-28
Volume
43
Issue
1
Pages
143-152
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2097096882
Cited by
55
References
51
Citations by year
2026: 4 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 13 · 2019: 2 · 2018: 8 · 2017: 6 · 2016: 2 · 2015: 2
FWCI
1.37
Type
journal article
Language
en
Open access
Locations
  • Nucleic Acids Research (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
Funders
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (820.02.013, 700.57.302)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
Keywords
TATA-binding protein, transcription termination, non-coding RNA, RNA polymerase II, preinitiation complex, transcriptional interference, Saccharomyces cerevisiae
MeSH terms
Adenosine Triphosphatases, Chromatin, Phosphoproteins, Promoter Regions, Genetic, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Gene Expression Regulation, Fungal, RNA, Antisense, RNA-Binding Proteins, RNA, Untranslated, 3' Flanking Region, Saccharomyces cerevisiae Proteins, TATA-Box Binding Protein, TATA-Binding Protein Associated Factors

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