Single-cell sequencing of the small-RNA transcriptome
Abstract
Little is known about the heterogeneity of small-RNA expression as small-RNA profiling has so far required large numbers of cells. Here we present a single-cell method for small-RNA sequencing and apply it to naive and primed human embryonic stem cells and cancer cells. Analysis of microRNAs and fragments of tRNAs and small nucleolar RNAs (snoRNAs) reveals the potential of microRNAs as markers for different cell types and states.
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tRFs have been implicated in neurological and neurodegenerative conditions including Alzheimer's disease, Parkinson's disease, psychological stress, and neuronal ferroptosis.
“tRFs have likewise been implicated in a growing number of neurological and neurodegenerative conditions 21,22 , including Alzheimer's disease 23,24 (AD), Parkinson's disease 25 (PD), psychological stress 26 , and neuronal ferroptosis 27 .”
From Human brain microRNAs exhibit cell-type specificity and age dependence (2026) · cites this paper as [22]
Some small ncRNAs are expressed in a tissue-, cell type-, or cell state-specific manner.
“with some being shown to be expressed in a tissue- (16), cell type- (17), or even cell state-specific manner (18)”
From A mouse tissue atlas of small noncoding RNA (2020) · cites this paper as [17]
Through their interactions with ribosomes and mRNA, small noncoding molecules shape the dynamic molecular spectrum of tissues.
“Through their interactions with ribosomes and mRNA, these small noncoding molecules shape the dynamic molecular spectrum of tissues (4, 17).”
From A mouse tissue atlas of small noncoding RNA (2020) · cites this paper as [17]
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Integrative single-cell analysis
Stuart, T., & Satija, R. (2019) · Nature Reviews Genetics · doi:10.1038/s41576-019-0093-7 · Cited by 1,460 · see citing works
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A mouse tissue atlas of small noncoding RNA
Isakova, A., Fehlmann, T., Keller, A., & Quake, S. R. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2002277117 · Cited by 119 · see citing works
Dori, M., Cavalli, D., Lesche, M., Massalini, S., Alieh, L. H. A., de Toledo, B. C., Khudayberdiev, S., Schratt, G., Dahl, A., & Calegari, F. (2020) · Development · doi:10.1242/dev.190520 · Cited by 22 · see citing works
Human brain microRNAs exhibit cell-type specificity and age dependence
Dubnov, S., Laski, L., Zchut, I., Avidan, O., Bennett, E. R., Greenberg, D. S., Turjeman, A., Nitzan, M., Paldor, I., & Soreq, H. (2026) · doi:10.64898/2026.01.04.697535
- Authors
- Faridani, O. R., Abdullayev, I., Hagemann-Jensen, M., Schell, J. P., Lanner, F., & Sandberg, R.
- Venue
- Nature Biotechnology
- Published
- 2016-10-31
- Volume
- 34
- Issue
- 12
- Pages
- 1264-1266
- ISSN
- 1087-0156
- 1546-1696
- Cited by
- 219
- References
- 6
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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