The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Free Radical-Independent Protection by Nerve Growth Factor and Bcl-2 of PC12 Cells from Hydrogen Peroxide-Triggered Apoptosis
Abstract
To investigate the mechanism of oxidative stress induced death of PC12 cells, we performed confocal and flow cytometric analysis with a reactive oxygen species (ROS)-specific fluorogen, 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHF-DA). Hydrogen peroxide significantly decreased the number of viable PC12 cells after 24 h. Hydrogen peroxide caused membrane blebbing, nuclear condensation and DNA fragmentation, indicating that the PC12 cells died due to apoptosis. The hydrogen peroxide-triggered apoptosis of PC12 cells was associated with enhanced ROS production in a dose-dependent manner by measuring with C-DCDHF-DA. Nerve growth factor (NGF) and Bcl-2 inhibited the hydrogen peroxide-induced apoptosis of PC12 cells. Neither of them, however, reduced the ROS production in PC12 cells. These data suggest that NGF or Bcl-2 protects PC12 cells from hydrogen peroxide-triggered apoptosis independently from ROS production.
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“| Ethanolic root extract | Parkinsonism | Mice | Increase in striatum dopamine | [306] |”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [306]
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“| Withaferin A | 3-NP induced HD | Rats | Increase in free radical scavenging activity | [53,306] |”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [306]
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Metadata
- Authors
- Satoh, T., Sakai, N., Enokido, Y., Uchiyama, Y., & Hatanaka, H.
- Affiliations
- The University of Osaka (JP)
- Institute for Protein Research, The University of Osaka (JP)
- Corresponding author
- Hatanaka, H.
- Venue
- Journal of Biochemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-09-01
- Volume
- 120
- Issue
- 3
- Pages
- 540-546
- ISSN
- 0021-924X (ISSN-L)
- OpenAlex
- W2023797581
- Cited by
- 146
- References
- 37
- Citations by year
- 2025: 1 · 2023: 6 · 2022: 3 · 2021: 2 · 2020: 5 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 5 · 2014: 4 · 2013: 7 · 2012: 6
- FWCI
- 2.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Neuroscience › Neurology › Neuroprotection in Cerebral Ischemia
- Keywords
- nerve growth factor, Bcl-2, PC12 cells, hydrogen peroxide, apoptosis, oxidative stress, reactive oxygen species, DNA fragmentation, membrane blebbing, neuroprotection
- MeSH terms
- Animals, Cell Membrane, Cell Survival, Flow Cytometry, Free Radicals, Hydrogen Peroxide, Kinetics, Nerve Growth Factors, Recombinant Proteins, Transfection, PC12 Cells, Apoptosis, Reactive Oxygen Species, Oxidative Stress, Microscopy, Confocal, Proto-Oncogene Proteins c-bcl-2, Rats
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