Cited by 74Lipid metabolism and biosynthesisCholesterol and Lipid MetabolismCancer, Lipids, and Metabolism

Complete Inhibition of Mouse Macrophage-Derived Foam Cell Formation by Triacsin C

Namatame, I., Tomoda, H., Arai, H., Inoue, K., Omura, S.·1999·Journal of Biochemistry

Abstract

Primary mouse peritoneal macrophages effectively take up and metabolize phosphatidylcholine/cholesterol liposomes containing a small amount of phosphatidylserine, which results in the massive accumulation in the cytoplasm of oil red O positive lipid droplets consisting of cholesteryl ester (CE) and triacylglycerol (TG) [Nishikawa et al. (1990) J. Biol. Chem. 265, 5226-5231]. A number of inhibitors of CE synthesis have been reported, but their effects on the lipid droplet formation have not been fully examined. Furthermore, the contribution of TG synthesis to lipid droplet formation has been poorly investigated. We have investigated the relationship between CE and TG syntheses and cytosolic lipid droplet formation in macrophages cultured in the presence of inhibitors with different modes of action. When macrophages were cultured with liposomes and [14C]oleic acid in the presence of triacsin C, a potent inhibitor of long chain acyl-CoA synthetase, both [14C]CE and [14C]TG syntheses were inhibited to similar extents with IC50 values of 0.19 and 0.10 microM, respectively. On the other hand, pregnenolone, a well-known inhibitor of cellular cholesterol transport, and CL-283,546, a potent inhibitor of acyl-CoA:cholesterol acyltransferase, inhibited [14C]CE synthesis specifically with IC50 values of 5.0 and 0.038 microM, respectively. Microscopic observation revealed that the inhibitors of cholesterol metabolism produce only a partial decrease in cytosolic lipid droplets even at the…

Integrity signals

Registry

Crossref · 10.1093/oxfordjournals.jbchem.a022289

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Treating iMGs with triacsin C effectively decreased BODIPY-labelled LDs.

    “Treating iMGs with triacsin C (TrC), an inhibitor of long-chain acyl-CoA synthetase that reduces LD formation10,18,19,39,40, effectively decreased BODIPY-labelled LDs (Extended Data Fig. 7e–i).”

Cites

Still being checked.

Cited by

Sorted by citations

Authors
Namatame, I., Tomoda, H., Arai, H., Inoue, K., & Omura, S.
Affiliations
  • Kitasato University (JP)
  • The University of Tokyo (JP)
Venue
Journal of Biochemistry
Published
1999-02-01
Volume
125
Issue
2
Pages
319-327
ISSN
0021-924X (ISSN-L)
OpenAlex
W2032401910
Cited by
74
References
0
Citations by year
2026: 2 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 4 · 2017: 1 · 2016: 5 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 2
FWCI
1.16
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repositories DataBase (IRDB) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Keywords
macrophage foam cell formation, lipid droplet formation, cholesteryl ester synthesis, triglyceride synthesis, acyl-CoA, acyl-CoA:cholesterol acyltransferase, peritoneal macrophages, atherosclerosis, neutral lipid synthesis, phosphatidylserine
MeSH terms
Long-Chain-Fatty-Acid-CoA Ligase, Animals, Carbon Radioisotopes, Cell Survival, Coenzyme A Ligases, Enzyme Inhibitors, Female, Foam Cells, Lipids, Liposomes, Mice, Inbred ICR, Phosphatidylcholines, Repressor Proteins, Streptomyces, Triazenes, Macrophages, Peritoneal, Oleic Acid, Saccharomyces cerevisiae Proteins, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review