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Cited by 157Rheumatoid Arthritis Research and TherapiesBone Metabolism and DiseasesOsteoarthritis Treatment and Mechanisms

Osteoprotegerin expression in synovial tissue from patients with rheumatoid arthritis, spondyloarthropathies and osteoarthritis and normal controls.

Haynes, D. R.·2003·Rheumatology
DOI 10.1093/rheumatology/keg047
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Abstract

OBJECTIVES: To demonstrate the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappaB ligand (RANKL) in synovial tissue from rheumatoid arthritis (RA) patients, establish the cell lineage expressing OPG and compare the expression of OPG in RA, spondyloarthropathies, osteoarthritis and normal synovial tissue. METHODS: Synovial biopsy specimens were obtained at arthroscopy from 16 RA and 12 spondyloarthropathy patients with active synovitis of a knee joint, six RA patients with no evidence of active synovitis, 10 patients with osteoarthritis and 18 normal subjects. Immunohistological analysis was performed using monoclonal antibodies (mAb) to detect OPG and RANKL expression. In addition, dual immunohistochemical evaluation was performed with lineage-specific monoclonal antibodies (macrophages, fibroblasts and endothelial cells) and OPG to determine the cell lineages expressing OPG. The sections were evaluated by computer-assisted image analysis and semiquantitative analysis. RESULTS: Two patterns of OPG expression were seen, one exclusively in endothelial cells and one expressed predominantly in macrophages in the synovial lining layer. Both patterns of OPG staining could be blocked with excess recombinant OPG. Endothelial and synovial lining expression of OPG was seen in all synovial tissues except those from patients with active RA. In contrast, RANKL expression was seen predominantly in synovial tissue from patients with active disease, mainly in sublining regions, particularly within areas of lymphocyte infiltration. CONCLUSIONS: OPG expression on macrophage type synovial lining cells as well as endothelial cells is deficient in RA patients with active synovitis, in contrast to that seen in spondyloarthropathy patients with active synovitis. This deficiency in OPG expression in the inflamed joint of RA patients may be important in the development of radiologically defined joint erosions.

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Authors
Haynes, D. R.
Affiliations
Repatriation General Hospital (AU)
Corresponding author
Haynes, D. R.
ORCID
Haynes, D. R.
Venue
Rheumatology
Publisher
Oxford University Press (OUP)
Published
2003-01-01
Volume
42
Issue
1
Pages
123-134
ISSN
  • 0263-7103 (ISSN-L)
  • 1460-2172
DOI
10.1093/rheumatology/keg047
OpenAlex
W2153911988
Cited by
157
References
31
Citations by year
2026: 1 · 2023: 3 · 2022: 2 · 2021: 5 · 2020: 3 · 2019: 7 · 2018: 3 · 2017: 6 · 2016: 5 · 2015: 8 · 2014: 8 · 2013: 9 · 2012: 4
FWCI
7.20
Type
journal article
Language
en
Open access
closed
Locations
  • British journal of rheumatology (publishedVersion)
  • PubMed (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
  • Flinders Academic Commons (Flinders University) (submittedVersion)
  • Pure Amsterdam UMC (submittedVersion)
Topics
  • Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
osteoprotegerin, RANKL, rheumatoid arthritis, spondyloarthritis, osteoarthritis, synovium, synovitis, macrophages, endothelial cells, synoviocytes, lymphocyte infiltration, joint erosions, bone resorption
MeSH terms
Acute Disease, Adult, Aged, Arthritis, Arthritis, Rheumatoid, Arthroscopy, Carrier Proteins, Endothelium, Female, Glycoproteins, Humans, Image Processing, Computer-Assisted, Immunohistochemistry, Knee Joint, Macrophages, Male, Membrane Glycoproteins, Middle Aged, Synovial Membrane, Blotting, Western, Case-Control Studies, Receptors, Tumor Necrosis Factor, Receptors, Cytoplasmic and Nuclear, Statistics, Nonparametric, Osteoarthritis, Knee, Spondylarthropathies, Osteoprotegerin, RANK Ligand, Receptor Activator of Nuclear Factor-kappa B

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