The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
RNAi-Mediated Resistance to Bean golden mosaic virus in Genetically Engineered Common Bean (Phaseolus vulgaris)
Open accessAbstract
Bean golden mosaic virus (BGMV) is transmitted by the whitefly Bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (Phaseolus vulgaris L.). The characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. The disease is the largest constraint to bean production in Latin America and causes severe yield losses (40 to 100%). Here, we explored the concept of using an RNA interference construct to silence the sequence region of the AC1 viral gene and generate highly resistant transgenic common bean plants. Eighteen transgenic common bean lines were obtained with an intron-hairpin construction to induce post-transcriptional gene silencing against the AC1 gene. One line (named 5.1) presented high resistance (approximately 93% of the plants were free of symptoms) upon inoculation at high pressure (more than 300 viruliferous whiteflies per plant during the whole plant life cycle) and at a very early stage of plant development. Transgene-specific small interfering RNAs were detected in both inoculated and non-inoculated transgenic plants. A semiquantitative polymerase chain reaction analysis revealed the presence of viral DNA in transgenic plants exposed to viruliferous whiteflies for a period of 6 days. However, when insects were removed, no virus DNA could be detected after an additional period of 6 days.
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Cites
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis
Edwards, K., Johnstone, C., & Thompson, C. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.6.1349 · Cited by 2,615
Construct design for efficient, effective and high‐throughput gene silencing in plants
Wesley, S. V., Helliwell, C. A., Smith, N. A., Wang, M., Rouse, D. T., Liu, Q., Gooding, P. S., Singh, S. P., Abbott, D., Stoutjesdijk, P. A., et al. (2001) · The Plant Journal · doi:10.1046/j.1365-313x.2001.01105.x · Cited by 1,532
Phytochromes and light signal perception by plants—an emerging synthesis
Smith, H. (2000) · Nature · doi:10.1038/35036500 · Cited by 1,380
Gene silencing as an adaptive defence against viruses
Waterhouse, P. M., Wang, M.-B., & Lough, T. (2001) · Nature · doi:10.1038/35081168 · Cited by 980
Total silencing by intron-spliced hairpin RNAs
Smith, N. A., Singh, S. P., Wang, M.-B., Stoutjesdijk, P. A., Green, A. G., & Waterhouse, P. M. (2000) · Nature · doi:10.1038/35030305 · Cited by 957
3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets
Birmingham, A., Anderson, E. M., Reynolds, A., Ilsley-Tyree, D., Leake, D., Fedorov, Y., Baskerville, S., Maksimova, E., Robinson, K., Karpilow, J., et al. (2006) · Nature Methods · doi:10.1038/nmeth854 · Cited by 920
Transcriptional silencing and promoter methylation triggered by double-stranded RNA
Mette, M. F., Aufsatz, W., van der Winden, J., Matzke, M. A., & Matzke, A. J. M. (2000) · The EMBO Journal · doi:10.1093/emboj/19.19.5194 · Cited by 869
The diversity of RNA silencing pathways in plants
BRODERSEN, P., & VOINNET, O. (2006) · Trends in Genetics · doi:10.1016/j.tig.2006.03.003 · Cited by 732
A Systemic Small RNA Signaling System in Plants
Yoo, B.-C., Kragler, F., Varkonyi-Gasic, E., Haywood, V., Archer-Evans, S., Lee, Y. M., Lough, T. J., & Lucas, W. J. (2004) · The Plant Cell · doi:10.1105/tpc.104.023614 · Cited by 528
Cited by
RNA interference technology in crop protection against arthropod pests, pathogens and nematodes
Zotti, M., dos Santos, E. A., Cagliari, D., Christiaens, O., Taning, C. N. T., & Smagghe, G. (2018) · Pest Management Science · doi:10.1002/ps.4813 · Cited by 465
Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives
Dong, O. X., & Ronald, P. C. (2019) · Plant Physiology · doi:10.1104/pp.18.01224 · Cited by 309
Ibrahim, A. B., Monteiro, T. R., Cabral, G. B., & Aragão, F. J. L. (2017) · Transgenic Research · doi:10.1007/s11248-017-0035-0 · Cited by 66
Self-citation
Metadata
- Authors
- Bonfim, K., Faria, J. C., Nogueira, E. O. P. L., Mendes, É. A., & Aragão, F. J. L.
- Venue
- Molecular Plant-Microbe Interactions®
- Publisher
- Scientific Societies
- Published
- 2007-06
- Volume
- 20
- Issue
- 6
- Pages
- 717-726
- ISSN
- 0894-0282 (ISSN-L)
- 1943-7706
- OpenAlex
- W1978726656
- Cited by
- 338
- References
- 25
- FWCI
- 11.55
- Type
- journal article
- Language
- en
- Open access
- green
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- Bonfim, K., Faria, J. C., Nogueira, E. O. P. L., Mendes, É. A., & Aragão, F. J. L. (2007). RNAi-Mediated Resistance to Bean golden mosaic virus in Genetically Engineered Common Bean (Phaseolus vulgaris). Molecular Plant-Microbe Interactions®. https://doi.org/10.1094/mpmi-20-6-0717
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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