The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
A Major Human Epididymis-Specific cDNA Encodes a Protein with Sequence Homology to Extracellular Proteinase Inhibitors1
Abstract
The amino acid sequence of a major human epididymis-specific protein was deduced from the nucleotide sequence of its cloned cDNA. The encoded product showed characteristics of a secretory protein, with a signal peptide followed by a small (approximately 10-kDa), acidic (pI 4.3), and cysteine-rich polypeptide. The positions of half-cysteines suggested that it was a two-domain member of the family of 'four-disulfide core' proteins to which a number of proteinase inhibitors belong. Southern blot analyses of human genomic DNA showed that the transcripts originated from a single copy gene. Northern blot and in situ transcript hybridization specifically localized the HE4 (human epididymis gene product) mRNA to the epithelial cells of the epididymal duct, predominantly within the distal sections. A possible function in sperm maturation as indicated by amino acid similarities to extracellular proteinase inhibitors of genital tract mucous secretions is discussed in the context of its tissue-specific transcription.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725
Church, G. M., & Gilbert, W. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.7.1991 · Cited by 8,116
Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA
CHEN, E. Y., & SEEBURG, P. H. (1985) · DNA · doi:10.1089/dna.1985.4.165 · Cited by 2,436
Patterns of Amino Acids near Signal‐Sequence Cleavage Sites
VON HEIJNE, G. (1983) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1983.tb07424.x · Cited by 2,280
Protein Inhibitors of Proteinases
Laskowski, M., & Kato, I. (1980) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.49.070180.003113 · Cited by 2,230
Selection of AUG initiation codons differs in plants and animals.
Lütcke, H. A., Chow, K. C., Mickel, F. S., Moss, K. A., Kern, H. F., & Scheele, G. A. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb04716.x · Cited by 1,030
Drenth, J., Low, B. W., Richardson, J. S., & Wright, C. S. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85783-6 · Cited by 158
Isolation and sequence of a human gene encoding a potent inhibitor of leukocyte proteases
Stetler, G. L., Brewer, M. T., & Thompson, R. C. (1986) · Nucleic Acids Research · doi:10.1093/nar/14.20.7883 · Cited by 140
Brooks, D. E., Means, A. R., Wright, E. J., et al. (1986) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1986.tb10118.x · Cited by 135
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Tumor biomarkers for diagnosis, prognosis and targeted therapy
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Comprehensive analysis of HE4 expression in normal and malignant human tissues
Galgano, M. T., Hampton, G. M., & Frierson, H. F. (2006) · Modern Pathology · doi:10.1038/modpathol.3800612 · Cited by 437
Identification of human epididymis protein-4 as a fibroblast-derived mediator of fibrosis
LeBleu, V. S., Teng, Y., O'Connell, J. T., Charytan, D., Müller, G. A., Müller, C. A., Sugimoto, H., & Kalluri, R. (2013) · Nature Medicine · doi:10.1038/nm.2989 · Cited by 260
Contribution of epididymal secretory proteins for spermatozoa maturation
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Metadata
- Authors
- Kirchhoff, C., Habben, I., Ivell, R., & Krull, N.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1991-08-01
- Volume
- 45
- Issue
- 2
- Pages
- 350-357
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W1981559854
- Cited by
- 395
- References
- 13
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A Major Human Epididymis-Specific cDNA Encodes a Protein with Sequence Homology to Extracellular Proteinase Inhibitors1. https://app.cochat.ai/papers/10.1095/biolreprod45.2.350
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- Kirchhoff, C., Habben, I., Ivell, R., & Krull, N. (1991). A Major Human Epididymis-Specific cDNA Encodes a Protein with Sequence Homology to Extracellular Proteinase Inhibitors1. Biology of Reproduction. https://doi.org/10.1095/biolreprod45.2.350
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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