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Cited by 377Reproductive Physiology in LivestockReproductive System and PregnancyReproductive Biology and Fertility

Endometrial Glands Are Required for Preimplantation Conceptus Elongation and Survival1

Gray, C. Allison, Taylor, Kristin M., Ramsey, W. Shawn, Hill, Jonathan R., Bazer, Fuller W., Bartol, Frank F., Spencer, Thomas E.·2001·Biology of Reproduction
DOI 10.1095/biolreprod64.6.1608
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Abstract

Endometrial glands secrete molecules hypothesized to support conceptus growth and development. In sheep, endometrial gland morphogenesis occurs postnatally and can be epigenetically ablated by neonatal progestin exposure. The resulting stable adult uterine gland knockout (UGKO) phenotype was used here to test the hypothesis that endometrial glands are required for successful pregnancy. Mature UGKO ewes were bred repeatedly to fertile rams, but no pregnancies were detected by ultrasound on Day 25. Day 7 blastocysts from normal superovulated ewes were then transferred synchronously into Day 7 control or UGKO ewes. Ultrasonography on Days 25-65 postmating indicated that pregnancy was established in control, but not in UGKO ewes. To examine early uterine-embryo interactions, four control and eight UGKO ewes were bred to fertile rams. On Day 14, their uteri were flushed. The uterus of each control ewe contained two filamentous conceptuses of normal length. Uteri from four UGKO ewes contained no conceptus. Uteri of three UGKO ewes contained a single severely growth-retarded tubular conceptus, whereas the remaining ewe contained a single filamentous conceptus. Histological analyses of these uteri revealed that endometrial gland density was directly related to conceptus survival and developmental state. Day 14 UGKO uteri that were devoid of endometrial glands did not support normal conceptus development and contained either no conceptuses or growth-retarded tubular conceptuses. The Day 14 UGKO uterus with moderate gland development contained a filamentous conceptus. Collectively, these results demonstrate that endometrial glands and, by inference, their secretions are required for periimplantation conceptus survival and development.

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Crossref · 10.1095/biolreprod64.6.1608

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  • Complete endocrine ablation of gland development in newborn lambs results in cessation of growth of the conceptus and loss of the pregnancy in the adult animal, whereas partial ablation leads to a small, non-expanded conceptus that fails to attach.

    “Complete ablation results in a cessation of growth of the conceptus and loss of the pregnancy in the adult animal, whereas partial ablation leads to a small, non-expanded conceptus that fails to attach.”

    From Placental Origins of Chronic Disease (2016) · cites this paper as [227]

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Authors
Gray, C. A., Taylor, K. M., Ramsey, W. S., Hill, J. R., Bazer, F. W., Bartol, F. F., & Spencer, T. E.
Affiliations
  • Texas A&M University (US)
  • Auburn University (US)
Corresponding author
Spencer, Thomas E.
ORCID
Venue
Biology of Reproduction
Publisher
Oxford University Press (OUP)
Published
2001-06-01
Volume
64
Issue
6
Pages
1608-1613
ISSN
  • 0006-3363 (ISSN-L)
  • 1529-7268
DOI
10.1095/biolreprod64.6.1608
OpenAlex
W2136960342
Cited by
377
References
48
Citations by year
2026: 17 · 2025: 14 · 2024: 9 · 2023: 19 · 2022: 12 · 2021: 30 · 2020: 19 · 2019: 16 · 2018: 27 · 2017: 13 · 2016: 18 · 2015: 13 · 2014: 18 · 2013: 15 · 2012: 16
FWCI
19.50
Type
journal article
Language
en
Open access
closed
Locations
  • Biology of Reproduction (publishedVersion)
  • PubMed (publishedVersion)
  • BioOne Complete (BioOne) (submittedVersion)
Funders
  • Sanofi
  • National Institute of Environmental Health Sciences (P30ES009106, P30‐ES09106)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
Keywords
endometrial glands, conceptus elongation, pregnancy establishment, sheep, blastocyst, uterine morphogenesis, uterine secretions
MeSH terms
Animals, Blastocyst, Drug Implants, Embryo Transfer, Endometrium, Female, Embryonic and Fetal Development, Interferon Type I, Pregnancy, Pregnancy Proteins, Pregnenediones, Progestins, Sheep, Superovulation, Dinoprost, Ultrasonography, Prenatal, Embryonic Development

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