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Cited by 140Erythropoietin and Anemia TreatmentPregnancy and preeclampsia studiesReproductive System and Pregnancy

Expression of erythropoietin by the human placenta

Conrad, Kirk P., Benyo, Deborah F., Westerhausen‐Larsen, Andrea, Miles, Theresa M.·1996·The FASEB Journal
DOI 10.1096/fasebj.10.7.8635693
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Abstract

Circulating levels of maternal erythro‐poietin (EPO) rise during gestation due to increased biosynthesis of the hormone. Our objective was to investigate the human placenta as a potential extrarenal site of EPO production. Using two mono‐clonal antibodies recognizing different antigenic determinants, we identified immunoreactive EPO associated with villous cytotrophoblast, endovascular and intravascular cytotrophoblast, cytotrophoblast cell columns, and syncytiotrophoblast of first and second‐trimester placenta as well as syncytiotro‐ phoblast and extravillous cytotrophoblast of normal third‐trimester and preeclamptic placenta. In addi‐tion, cultured JAR (trophoblast‐derived) choriocar‐cinoma cells, cytotrophoblasts isolated from term placenta, villous core cells, and possibly other non‐ trophoblast cells within the decidual basal plate ex‐pressed immunoreactive EPO. Using reverse transcription‐polymerase chain reaction and EPO‐ specific primers, a 378 bp DNA product was ampli‐fied from placental tissues of various gestational ages, cytotrophoblasts isolated from term placenta, and JAR choriocarcinoma cells. The amplified product yielded restriction enzyme fragments of predicted sizes. On Southern analysis, hybridization was observed for two of these fragments in which the radiolabeled EPO cDNA probe did not overlap with the primer sequences. Finally, the JAR choriocarcinoma cells elaborated EPO into the culture medium as determined by enzyme‐linked immunosorbant assay and expressed EPO mRNA as determined by Northern analysis, both of which were stimulated by hypoxia (15‐20 torr). Taken together, these results suggest a new site of EPO expression: the trophoblast cell of the human placenta.—Conrad, K. P., Benyo, D. F., Westerhausen‐Larsen, A., Miles, T. M. Expression of erythropoietin by the human placenta. FASEB J. 10, 760‐766 (1996)

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  • The placenta secretes erythropoietin, angiotensin II, and adrenomedullin, hormones traditionally associated with the hypoxic kidney.

    “Finally, it is important to note that the placenta secretes a number of hormones that are traditionally associated with the hypoxic kidney, including erythropoietin, angiotensin II and adrenomedullin (123, 357).”

    From Placental Origins of Chronic Disease (2016) · cites this paper as [123]

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Authors
Conrad, K. P., Benyo, D. F., Westerhausen‐Larsen, A., & Miles, T. M.
Affiliations
  • University of Pittsburgh (US)
  • Magee‐Womens Research Institute and Department of Obstetrics, Gynecology and Reproductive Sciences University of Pittsburgh Pittsburgh Pennsylvania 15213 USA
Corresponding author
Conrad, Kirk P.
ORCID
Conrad, Kirk P.
Venue
The FASEB Journal
Publisher
Wiley
Published
1996-05
Volume
10
Issue
7
Pages
760-768
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
DOI
10.1096/fasebj.10.7.8635693
OpenAlex
W1933030459
Cited by
138
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2023: 1 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 3 · 2012: 8
FWCI
2.83
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • The FASEB Journal (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5k04hd001098-02)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD30325, R01HD030325, K04 HD-01098)
Topics
  • Health Sciences › Medicine › Hematology › Erythropoietin and Anemia Treatment
  • Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
Keywords
erythropoietin, placenta, trophoblast, cytotrophoblast, syncytiotrophoblast, extravillous cytotrophoblast, JAR choriocarcinoma cells, hypoxia, preeclampsia
MeSH terms
Enzyme-Linked Immunosorbent Assay, Erythropoietin, Female, Humans, Immunohistochemistry, Placenta, RNA, Messenger, Polymerase Chain Reaction

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