The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Chronic stretch of engineered heart tissue induces hypertrophy and functional improvement
Abstract
To examine the influence of chronic mechanical stretch on functional behavior of cardiac myocytes, we reconstituted embryonic chick or neonatal rat cardiac myocytes to a 3‐dimensional engineered heart tissue (EHT) by mixing freshly isolated cells with neutralized collagen I and culturing them between two Velcro‐coated silicone tubes, held at a fixed distance with a metal spacer. After 4 days, EHTs were subjected to a phasic unidirectional stretch for 6 days in serum‐containing medium. Compared to unstretched controls, RNA/DNA and protein/cell ratios increased by 100% and 50%, respectively. ANF mRNA and a‐sarcomeric actin increased by 98% and 40%, respectively. Morphologically, stretched EHTs exhibited improved organization of cardiac myocytes into parallel arrays of rod‐shaped cells, increased cell length and width, longer myofilaments, and increased mitochondrial density. Thus, stretch induced phenotypic changes, generally referred to as hypertrophy. Concomitantly, force of contraction was two‐ to fourfold higher both under basal conditions and after stimulation with calcium or the β‐adrenergic agonist isoprenaline. Contraction kinetics were accelerated with a 14–44% decrease in twitch duration under all those conditions. In summary, we have developed a new in vitro model that allows morphological, molecular, and functional consequences of stretch to be studied under defined conditions. The main finding was that stretch of EHTs induced cardiac myocyte hypertrophy, which was accompanied by marked improvement of contractile function.—Fink, C., Ergün, S., Kralisch, D., Remmers, U., Weil, J., Eschenhagen, T. Chronic stretch of engineered heart tissue induces hypertrophy and functional improvement. FASEB J. 14, 669–679 (2000)
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Levy, D., Garrison, R. J., Savage, D. D., Kannel, W. B., & Castelli, W. P. (1990) · New England Journal of Medicine · doi:10.1056/nejm199005313222203 · Cited by 5,699
A Calcineurin-Dependent Transcriptional Pathway for Cardiac Hypertrophy
Molkentin, J. D., Lu, J.-R., Antos, C. L., Markham, B., Richardson, J., Robbins, J., Grant, S. R., & Olson, E. N. (1998) · Cell · doi:10.1016/s0092-8674(00)81573-1 · Cited by 2,566
Molecular Mechanisms of Myocardial Remodeling
SWYNGHEDAUW, B. (1999) · Physiological Reviews · doi:10.1152/physrev.1999.79.1.215 · Cited by 1,460
THE CELLULAR AND MOLECULAR RESPONSE OF CARDIAC MYOCYTES TO MECHANICAL STRESS
Sadoshima, J., & Izumo, S. (1997) · Annual Review of Physiology · doi:10.1146/annurev.physiol.59.1.551 · Cited by 864
Eschenhagen, T., Fink, C., Remmers, U., Scholz, H., Wattchow, J., Weil, J., Zimmermann, W., Dohmen, H. H., Schäfer, H., Bishopric, N., et al. (1997) · The FASEB Journal · doi:10.1096/fasebj.11.8.9240969 · Cited by 636
Self-citationTransgenic Gαq overexpression induces cardiac contractile failure in mice
D’Angelo, D. D., Sakata, Y., Lorenz, J. N., Boivin, G. P., Walsh, R. A., Liggett, S. B., & Dorn, G. W. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.15.8121 · Cited by 629
Simpson, P. (1983) · Journal of Clinical Investigation · doi:10.1172/jci111023 · Cited by 618
Sadoshima, J., Jahn, L., Takahashi, T., Kulik, T. J., & Izumo, S. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50052-7 · Cited by 605
Cited by
Tissue Engineering of a Differentiated Cardiac Muscle Construct
Zimmermann, W.-H., Schneiderbanger, K., Schubert, P., Didié, M., Münzel, F., Heubach, J. F., Kostin, S., Neuhuber, W. L., & Eschenhagen, T. (2002) · Circulation Research · doi:10.1161/hh0202.103644 · Cited by 917
Self-citationAccordion-like honeycombs for tissue engineering of cardiac anisotropy
Engelmayr, G. C., Cheng, M., Bettinger, C. J., Borenstein, J. T., Langer, R., & Freed, L. E. (2008) · Nature Materials · doi:10.1038/nmat2316 · Cited by 848
Growth of engineered human myocardium with mechanical loading and vascular coculture
Tulloch, N. L., Muskheli, V., Razumova, M. V., Korte, F. S., Regnier, M., Hauch, K. D., Pabon, L., Reinecke, H., & Murry, C. E. (2011) · Circulation Research · doi:10.1161/circresaha.110.237206 · Cited by 666
Nanoscale cues regulate the structure and function of macroscopic cardiac tissue constructs
Kim, D.-H., Lipke, E. A., Kim, P., Cheong, R., Thompson, S., Delannoy, M., Suh, K.-Y., Tung, L., & Levchenko, A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906504107 · Cited by 611
Development of a drug screening platform based on engineered heart tissue
Hansen, A., Eder, A., Bönstrup, M., Flato, M., Mewe, M., Schaaf, S., Aksehirlioglu, B., Schwörer, A., Uebeler, J., & Eschenhagen, T. (2010) · Circulation Research · doi:10.1161/circresaha.109.211458 · Cited by 510
Metadata
- Authors
- Fink, C., Ergün, S., Kralisch, D., Remmers, U., Weil, J., & Eschenhagen, T.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2000-04
- Volume
- 14
- Issue
- 5
- Pages
- 669-679
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1522672235
- Cited by
- 377
- References
- 32
- FWCI
- 12.29
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Chronic stretch of engineered heart tissue induces hypertrophy and functional improvement. https://app.cochat.ai/papers/10.1096/fasebj.14.5.669
- The paper
- Fink, C., Ergün, S., Kralisch, D., Remmers, U., Weil, J., & Eschenhagen, T. (2000). Chronic stretch of engineered heart tissue induces hypertrophy and functional improvement. The FASEB Journal. https://doi.org/10.1096/fasebj.14.5.669
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
