The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20‐hydroxy‐ and 20,23‐dihydroxyvitamin D
Open accessAbstract
RORα and RORγ are expressed in human skin cells that produce the noncalcemic 20‐hydroxyvitamin D 3 [20(OH)D 3 ] and 20,23‐dihydroxyvitamin D 3 [20,23(OH) 2 D 3 ]. Chinese hamster ovary (CHO) cells stably expressing a Tet‐on RORα or RORγ expression vector and a ROR‐responsive element (RORE)‐LUC reporter, and a mammalian 2‐hybrid model examining the interaction between the ligand binding domain (LBD) of RORα or RORγ with an LBD‐interacting LXXLL‐peptide, were used to study ROR‐antagonist activities. These assays revealed that 20(OH)D 3 and 20,23(OH) 2 D 3 function as antagonists of RORα and RORγ. Moreover, 20(OH)D 3 inhibited the activation of the promoter of the Bmal1 and G6pase genes, targets of RORα, and 20(OH)D 3 and 20,23(OH) 2 D 3 inhibited Il17 promoter activity in Jurkat cells overexpressing RORα or RORγ. Molecular modeling using crystal structures of the LBDs of RORα and RORγ revealed docking scores for 20(OH)D 3 , 20, 23(OH) 2 D 3 and 1,25(OH) 2 D 3 similar to those of the natural ligands, predicting good binding to the receptor. Notably, 20(OH)D 3 , 20,23(OH) 2 D 3 , and 1,25(OH) 2 D 3 inhibited RORE‐mediated activation of a reporter in keratinocytes and melanoma cells and inhibited IL‐17 production by immune cells. Our study identifies a novel signaling pathway, in which 20(OH)D 3 and 20,23(OH) 2 D 3 act as antagonists or inverse agonists of RORα and RORγ, that opens new possibilities for local (skin) or systemic regulation.—Slominski, A. T., Kim, T.‐K., Takeda, Y., Janjetovic, Z., Brożyna, A. A., Skobowiat, C., Wang, J., Postlethwaite, A., Li, W., Tuckey, R. C., Jetten, A. M. RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20‐hydroxy‐ and 20,23‐dihydroxyvitamin D. FASEB J . 28, 2775–2789 (2014). www.fasebj.org
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Holick, M. F. (2007) · New England Journal of Medicine · doi:10.1056/nejmra070553 · Cited by 13,720
The cutaneous serotoninergic/melatoninergic system: securing a place under the sun
Slominski, A., Wortsman, J., & Tobin, D. J. (2005) · The FASEB Journal · doi:10.1096/fj.04-2079rev · Cited by 402
Self-citationCutaneous hypothalamic–pituitary–adrenal axis homolog: regulation by ultraviolet radiation
Skobowiat, C., Dowdy, J. C., Sayre, R. M., Tuckey, R. C., & Slominski, A. (2011) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00217.2011 · Cited by 269
Self-citationSlominski, A. T., Kim, T., Shehabi, H. Z., Semak, I., Tang, E. K. Y., Nguyen, M. N., Benson, H. A. E., Korik, E., Janjetovic, Z., Chen, J., et al. (2012) · The FASEB Journal · doi:10.1096/fj.12-208975 · Cited by 266
Self-citationThe ROR nuclear orphan receptor subfamily: Critical regulators of multiple biological processes
Jetten, A. M., Kurebayashi, S., & Ueda, E. (2001) · Progress in Nucleic Acid Research and Molecular Biology · doi:10.1016/s0079-6603(01)69048-2 · Cited by 232
Self-citationExpression of vitamin D receptor decreases during progression of pigmented skin lesions
Brożyna, A. A., Jozwicki, W., Janjetovic, Z., & Slominski, A. T. (2011) · Human Pathology · doi:10.1016/j.humpath.2010.09.014 · Cited by 120
Self-citationPathways and products for the metabolism of vitamin D3 by cytochrome P450scc
Tuckey, R. C., Li, W., Zjawiony, J. K., Zmijewski, M. A., Nguyen, M. N., Sweatman, T., Miller, D., & Slominski, A. (2008) · The FEBS Journal · doi:10.1111/j.1742-4658.2008.06406.x · Cited by 118
Self-citationSkobowiat, C., Sayre, R. M., Dowdy, J. C., & Slominski, A. T. (2013) · British Journal of Dermatology · doi:10.1111/bjd.12096 · Cited by 115
Self-citationSlominski, A. T., Kim, T.-K., Janjetovic, Z., Tuckey, R. C., Bieniek, R., Yue, J., Li, W., Chen, J., Nguyen, M. N., Tang, E. K. Y., et al. (2011) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00203.2010 · Cited by 113
Self-citationSlominski, A. T., Kim, T.-K., Shehabi, H. Z., Tang, E. K. Y., Benson, H. A. E., Semak, I., Lin, Z., Yates, C. R., Wang, J., Li, W., et al. (2014) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2013.12.012 · Cited by 100
Slominski, A., Semak, I., Wortsman, J., Zjawiony, J., Li, W., Zbytek, B., & Tuckey, R. C. (2006) · The FEBS Journal · doi:10.1111/j.1742-4658.2006.05302.x · Cited by 98
Self-citationSlominski, A., Janjetovic, Z., Tuckey, R. C., Nguyen, M. N., Bhattacharya, K. G., Wang, J., Li, W., Jiao, Y., Gu, W., Brown, M., et al. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2012-3074 · Cited by 89
Self-citationTuckey, R. C., Li, W., Shehabi, H. Z., Janjetovic, Z., Nguyen, M. N., Kim, T.-K., Chen, J., Howell, D. E., Benson, H. A. E., Sweatman, T., et al. (2011) · Drug Metabolism and Disposition · doi:10.1124/dmd.111.040071 · Cited by 88
Self-citationSlominski, A. T., Kim, T.-K., Chen, J., Nguyen, M. N., Li, W., Yates, C. R., Sweatman, T., Janjetovic, Z., & Tuckey, R. C. (2012) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2012.07.027 · Cited by 81
Self-citationVitamin D analogs 17, 20S(OH)2pD and 17,20R(OH)2pD are noncalcemic and exhibit antifibrotic activity
Slominski, A. T., Li, W., Bhattacharya, S. K., Smith, R. A., Johnson, P. L., Chen, J., Nelson, K. E., Tuckey, R. C., Miller, D., Jiao, Y., et al. (2011) · Journal of Investigative Dermatology · doi:10.1038/jid.2010.425 · Cited by 51
Self-citation
Cited by
Melatonin and inflammation-Story of a double-edged blade
Hardeland, R. (2018) · Journal of Pineal Research · doi:10.1111/jpi.12525 · Cited by 455
Aging, melatonin, and the pro- and anti-inflammatory networks
Hardeland, R. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20051223 · Cited by 337
Novel activities of CYP11A1 and their potential physiological significance
Slominski, A. T., Li, W., Kim, T.-K., Semak, I., Wang, J., Zjawiony, J. K., & Tuckey, R. C. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2014.11.010 · Cited by 292
Self-citationThe serum vitamin D metabolome: what we know and what is still to discover
Tuckey, R. C., Cheng, C. Y. S., & Slominski, A. T. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2018.09.003 · Cited by 245
Self-citationChaiprasongsuk, A., Janjetovic, Z., Kim, T.-K., Jarrett, S. G., D'Orazio, J. A., Holick, M. F., Tang, E. K. Y., Tuckey, R. C., Panich, U., Li, W., et al. (2019) · Redox Biology · doi:10.1016/j.redox.2019.101206 · Cited by 146
Self-citation
Metadata
- Authors
- Slominski, A. T., Kim, T., Takeda, Y., Janjetovic, Z., Brożyna, A. A., Skobowiat, C., Wang, J., Postlethwaite, A., Li, W., Tuckey, R. C., & Jetten, A. M.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2014-03-25
- Volume
- 28
- Issue
- 7
- Pages
- 2775-2789
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1978537670
- Cited by
- 269
- References
- 15
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
